Sharma S, Mehta S, Morgan J, Maizel A
Science. 1987 Mar 20;235(4795):1489-92. doi: 10.1126/science.3547651.
A human B-cell growth factor (BCGF) (12 kilodaltons) supports the clonal proliferation of B lymphocytes. A clone was isolated that contained the proper structural sequence to encode biologically active, 12-kilodalton BCGF in Escherichia coli and to hybridize to a specific messenger RNA, identified by in vitro translation in Xenopus laevis oocytes. A relatively hydrophobic region of 18 amino acids was found at the amino terminal of the 124-amino acid-long polypeptide. The carboxyl terminal is composed of at least 32 amino acids that are derived from nucleotide sequences bearing significant homology to the Alu repeat family.
一种人类B细胞生长因子（BCGF，12千道尔顿）可支持B淋巴细胞的克隆增殖。分离出了一个克隆，该克隆包含在大肠杆菌中编码具有生物活性的12千道尔顿BCGF的正确结构序列，并能与通过非洲爪蟾卵母细胞体外翻译鉴定的特定信使RNA杂交。在这个124个氨基酸长的多肽的氨基末端发现了一个由18个氨基酸组成的相对疏水区域。羧基末端由至少32个氨基酸组成，这些氨基酸源自与Alu重复序列家族具有显著同源性的核苷酸序列。
