铜诱导细胞因子与酵母金属硫蛋白上游激活序列的结合。
Copper-induced binding of cellular factors to yeast metallothionein upstream activation sequences.
作者信息
Huibregtse J M, Engelke D R, Thiele D J
机构信息
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109-0606.
出版信息
Proc Natl Acad Sci U S A. 1989 Jan;86(1):65-9. doi: 10.1073/pnas.86.1.65.
Copper-inducible transcription of the yeast metallothionein gene (CUP1) occurs by means of cis-acting upstream activation sequences (UAS) and trans-acting cellular factors. We have used a high-resolution chromosomal footprinting technique to detect the interaction of cellular factors with UASCUP1. Our results demonstrate that one or more cellular factors bind to UASCUP1 in a copper-inducible fashion. This copper-inducible binding is enhanced in a yeast strain that harbors several copies of the positive regulatory gene ACE1 and is not detectable in yeast cells that contain a nonfunctional (ace1-delta 1) locus. The correlation between yeast metallothionein gene activation and copper-inducible occupation of UASCUP1 sequences suggests that the binding of metallothionein transcriptional regulatory factors to cis-acting control sequences is copper-inducible.
酵母金属硫蛋白基因（CUP1）的铜诱导转录是通过顺式作用上游激活序列（UAS）和反式作用细胞因子实现的。我们使用了一种高分辨率染色体足迹技术来检测细胞因子与UASCUP1的相互作用。我们的结果表明，一种或多种细胞因子以铜诱导的方式与UASCUP1结合。在携带多个阳性调节基因ACE1拷贝的酵母菌株中，这种铜诱导结合增强，而在含有无功能（ace1-delta 1）位点的酵母细胞中则无法检测到。酵母金属硫蛋白基因激活与UASCUP1序列的铜诱导占据之间的相关性表明，金属硫蛋白转录调节因子与顺式作用控制序列的结合是铜诱导的。
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