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在大肠杆菌中产生的ACE1转录因子与酵母金属硫蛋白上游激活序列内的多个区域结合。

ACE1 transcription factor produced in Escherichia coli binds multiple regions within yeast metallothionein upstream activation sequences.

作者信息

Evans C F, Engelke D R, Thiele D J

机构信息

Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109-0606.

出版信息

Mol Cell Biol. 1990 Jan;10(1):426-9. doi: 10.1128/mcb.10.1.426-429.1990.

DOI:10.1128/mcb.10.1.426-429.1990
PMID:2403647
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360775/
Abstract

The ACE1 protein of Saccharomyces cerevisiae was expressed as a trpE-ACE1 fusion protein in Escherichia coli and shown to bind CUP1 upstream activation sequences at multiple regions in a copper-inducible manner. These binding sites contain within them the sequence 5'-TC(T)4-6GCTG-3', which we propose constitutes an important part of the ACE1 consensus recognition sequence.

摘要

酿酒酵母的ACE1蛋白在大肠杆菌中作为trpE-ACE1融合蛋白表达，并被证明以铜诱导方式在多个区域结合CUP1上游激活序列。这些结合位点包含序列5'-TC(T)4-6GCTG-3'，我们认为该序列构成了ACE1共有识别序列的重要部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0d1/360775/3efb1188ea1a/molcellb00037-0448-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0d1/360775/26c06910c88c/molcellb00037-0447-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0d1/360775/3efb1188ea1a/molcellb00037-0448-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0d1/360775/26c06910c88c/molcellb00037-0447-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0d1/360775/3efb1188ea1a/molcellb00037-0448-a.jpg

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ACE1 transcription factor produced in Escherichia coli binds multiple regions within yeast metallothionein upstream activation sequences.在大肠杆菌中产生的ACE1转录因子与酵母金属硫蛋白上游激活序列内的多个区域结合。
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本文引用的文献

1
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Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.编码转录因子Sp1的cDNA的分离及DNA结合结构域的功能分析。
Cell. 1987 Dec 24;51(6):1079-90. doi: 10.1016/0092-8674(87)90594-0.
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Yeast metallothionein and applications in biotechnology.
弱酸性阴离子在酿酒酵母中通过差异结合激活 Haa1 和 War1 转录因子。
Nucleic Acids Res. 2019 Feb 20;47(3):1211-1224. doi: 10.1093/nar/gky1188.
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Genome-wide analysis of the regulation of Cu metabolism in Cryptococcus neoformans.全基因组分析新型隐球菌铜代谢调控。
Mol Microbiol. 2018 Jun;108(5):473-494. doi: 10.1111/mmi.13960. Epub 2018 Apr 24.
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Copper Acquisition and Utilization in Fungi.真菌中的铜获取和利用。
Annu Rev Microbiol. 2017 Sep 8;71:597-623. doi: 10.1146/annurev-micro-030117-020444.
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The Zygosaccharomyces bailii transcription factor Haa1 is required for acetic acid and copper stress responses suggesting subfunctionalization of the ancestral bifunctional protein Haa1/Cup2.巴氏接合酵母转录因子Haa1是乙酸和铜胁迫应答所必需的，这表明祖先双功能蛋白Haa1/Cup2发生了亚功能化。
BMC Genomics. 2017 Jan 13;18(1):75. doi: 10.1186/s12864-016-3443-2.
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Regulation of cation balance in Saccharomyces cerevisiae.酵母中阳离子平衡的调节。
Genetics. 2013 Mar;193(3):677-713. doi: 10.1534/genetics.112.147207.
8
Identification of negative cis-acting elements in response to copper in the chloroplastic iron superoxide dismutase gene of the moss Barbula unguiculata.藓类植物小扭口藓叶绿体铁超氧化物歧化酶基因中响应铜的负向顺式作用元件的鉴定
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10
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Proc Natl Acad Sci U S A. 1989 Jan;86(1):65-9. doi: 10.1073/pnas.86.1.65.