Jin Yan, Wong Kok Hou, Granville Anthony Michael
Centre for Advanced Macromolecular Design, School of Chemical Engineering, University of New South Wales, Sydney, Australia.
Centre for Advanced Macromolecular Design, School of Chemical Engineering, University of New South Wales, Sydney, Australia.
J Colloid Interface Sci. 2016 Jan 15;462:19-28. doi: 10.1016/j.jcis.2015.09.047. Epub 2015 Sep 21.
Poly(methyl methacrylate) polymer based Localized Surface Plasmon Resonance biosensor chips were successfully fabricated using glycopolymer brushes carrying glucose moieties for the detection of concanavalin A. Poly(pentafluorostyrene), with pre-determined polymer chain lengths, were synthesized via a reversible addition-fragmentation chain transfer polymerization technique. The synthesized poly(pentafluorostyrene), was subsequently converted into glycopolymers via a para-fluoro-thiol "click" reaction and grafted onto the surface of sensor chips. The "glycocluster effect" induced by pendent carbohydrate moieties enabled a stronger affinity for concanavalin A binding, which resulted in a dramatic expansion of the sensors' response range. It was discovered that the longer polymer brushes did not guarantee additional enhancements for the sensor chips. Instead, they could lead to higher detection limits. In this study, the limit of detection for the sensor chips was discovered to be 1.3nmolL(-1) with a saturated response at 1054.2nmolL(-1). In addition to the superior performance, the capabilities of the reported sensor chips can be easily manipulated to detect a diverse range of analytes by "clicking" various sensing elements onto the polymer brushes.
基于聚甲基丙烯酸甲酯聚合物的局域表面等离子体共振生物传感器芯片已成功制备，该芯片使用带有葡萄糖部分的糖聚合物刷来检测伴刀豆球蛋白A。通过可逆加成-断裂链转移聚合技术合成了具有预定聚合物链长度的聚五氟苯乙烯。随后，通过对氟硫醇“点击”反应将合成的聚五氟苯乙烯转化为糖聚合物，并接枝到传感器芯片表面。由悬垂碳水化合物部分诱导的“糖簇效应”使得对伴刀豆球蛋白A结合具有更强的亲和力，这导致传感器响应范围显著扩大。研究发现，较长的聚合物刷并不能保证传感器芯片有额外的增强效果。相反，它们可能导致更高的检测限。在本研究中，发现传感器芯片的检测限为1.3nmolL(-1)，在1054.2nmolL(-1)时达到饱和响应。除了卓越的性能外，通过将各种传感元件“点击”到聚合物刷上，所报道的传感器芯片的功能可以很容易地进行操作，以检测各种分析物。
