AIST-Osaka University Advanced Photonics and Biosensing Open Innovation Laboratory, AIST, Suita, Osaka 565-0871, Japan.
Department of Applied Physics, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
ACS Appl Bio Mater. 2021 Nov 15;4(11):7913-7920. doi: 10.1021/acsabm.1c00834. Epub 2021 Oct 28.
A Au-capped nanopillar chip was prepared using nanoimprint lithography (NIL) and Au sputtering onto a cyclo-olefin polymer film. The Au surface of the chip exerting localized surface plasmon resonance (LSPR) phenomena was immobilized with a glycopolymer for the detection of cytokines. The glycopolymers were synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization for controlled polymer chain length, and thiol-terminated glycopolymers with chain lengths of 20-, 100-, and 200-mers were designed. The thickness of the biomolecular layer on the Au surface was controlled by changing the polymer chain length of the immobilized glycopolymer, and the absorption of proteins onto the Au surface was detected by the shift of absorbance peak wavelength. The value of absorbance peak wavelength shift by protein adsorption increased as the glycopolymer layer thickness became thinner. This difference in LSPR signal response was remarkable for cytokine recognition compared to larger proteins. It was shown that controlling the biomolecular layer thickness was effective for the detection of small proteins, and our research suggested the usefulness of the controlled glycopolymer surface as a molecular recognition material for cytokine detection.
采用纳米压印光刻（NIL）和金溅射技术在环烯烃聚合物薄膜上制备了 Au 封端纳米柱芯片。芯片上的 Au 表面通过固定糖聚合物来检测细胞因子，从而表现出局域表面等离子体共振（LSPR）现象。糖聚合物通过可逆加成-断裂链转移（RAFT）聚合合成，以控制聚合物链长，设计了链长为 20、100 和 200 个重复单元的巯基封端糖聚合物。通过改变固定化糖聚合物的聚合物链长来控制 Au 表面上生物分子层的厚度，并通过吸光度峰波长的移动来检测蛋白质在 Au 表面上的吸收。随着糖聚合物层厚度变薄，蛋白质吸附引起的吸光度峰波长移动值增加。与较大的蛋白质相比，这种 LSPR 信号响应的差异对于细胞因子识别非常显著。结果表明，控制生物分子层厚度对于检测小蛋白非常有效，我们的研究表明，控制糖聚合物表面作为细胞因子检测的分子识别材料是有用的。
