Yao Shao-Xin, Zhang Gui-Song, Cao Hong-Xia, Song Guang, Li Zang-Tuo, Zhang Wei-Tao
Department of Interventional Therapy, Affiliated Tangshan Workers Hospital of Hebei Medical University, Tangshan, Hebei, 063000, China.
Department of Interventional Therapy, Luanxian People's Hospital, Tangshan, Hebei, 063700, China.
Asian Pac J Trop Med. 2015 Sep;8(9):762-5. doi: 10.1016/j.apjtm.2015.07.021. Epub 2015 Jul 29.
To study the correlation between miR-21 and Treg/Th17 ratio in the microenvironment of rats with hepatocellular carcinoma.
Diethylnitrosamine was used to build the hepatocellular carcinoma model of rats; the content of Treg cells and Th17 cells and the expression of miR-21 in the peripheral blood of rats with hepatocellular carcinoma were detected. The statistical analysis was performed on the correlation between miR-21 expression and Treg/Th17 ratio.
Hepatocellular carcinoma model of rats was successfully constructed. The proportion of Th17 cells among all CD4(+)T cells in the peripheral blood of rats with hepatocellular carcinoma was 5.319%, which was higher than the control group; while the proportion of Treg cells was 9.472%, which was higher than the control group. Treg/Th17 ratio in the model group was 1.781, compared with 1.478 in the control group. The expression of miR-21 was increased in the peripheral blood of rats with hepatocellular carcinoma and it showed a positive correlation with the ratio of Treg/Th17.
There is a positive correlation between the expression level of miR-21 and the ratio of Treg/Th17.
研究肝细胞癌大鼠微环境中miR-21与Treg/Th17比值的相关性。
采用二乙基亚硝胺建立大鼠肝细胞癌模型；检测肝细胞癌大鼠外周血中Treg细胞和Th17细胞的含量以及miR-21的表达。对miR-21表达与Treg/Th17比值之间的相关性进行统计分析。
成功构建大鼠肝细胞癌模型。肝细胞癌大鼠外周血中所有CD4(+)T细胞中Th17细胞的比例为5.319%，高于对照组；而Treg细胞的比例为9.472%，高于对照组。模型组Treg/Th17比值为1.781，对照组为1.478。miR-21在肝细胞癌大鼠外周血中的表达升高，且与Treg/Th17比值呈正相关。
miR-21表达水平与Treg/Th17比值呈正相关。
