肝细胞癌大鼠微环境中微小RNA-21与Th17和Treg细胞表达的相关性

Correlation between microRNA-21 and expression of Th17 and Treg cells in microenvironment of rats with hepatocellular carcinoma.

作者信息

Yao Shao-Xin, Zhang Gui-Song, Cao Hong-Xia, Song Guang, Li Zang-Tuo, Zhang Wei-Tao

机构信息

Department of Interventional Therapy, Affiliated Tangshan Workers Hospital of Hebei Medical University, Tangshan, Hebei, 063000, China.

Department of Interventional Therapy, Luanxian People's Hospital, Tangshan, Hebei, 063700, China.

出版信息

Asian Pac J Trop Med. 2015 Sep;8(9):762-5. doi: 10.1016/j.apjtm.2015.07.021. Epub 2015 Jul 29.

DOI:10.1016/j.apjtm.2015.07.021

PMID:26433664

Abstract

OBJECTIVE

To study the correlation between miR-21 and Treg/Th17 ratio in the microenvironment of rats with hepatocellular carcinoma.

METHODS

Diethylnitrosamine was used to build the hepatocellular carcinoma model of rats; the content of Treg cells and Th17 cells and the expression of miR-21 in the peripheral blood of rats with hepatocellular carcinoma were detected. The statistical analysis was performed on the correlation between miR-21 expression and Treg/Th17 ratio.

RESULTS

Hepatocellular carcinoma model of rats was successfully constructed. The proportion of Th17 cells among all CD4(+)T cells in the peripheral blood of rats with hepatocellular carcinoma was 5.319%, which was higher than the control group; while the proportion of Treg cells was 9.472%, which was higher than the control group. Treg/Th17 ratio in the model group was 1.781, compared with 1.478 in the control group. The expression of miR-21 was increased in the peripheral blood of rats with hepatocellular carcinoma and it showed a positive correlation with the ratio of Treg/Th17.

CONCLUSIONS

There is a positive correlation between the expression level of miR-21 and the ratio of Treg/Th17.

摘要

目的

研究肝细胞癌大鼠微环境中miR-21与Treg/Th17比值的相关性。

方法

采用二乙基亚硝胺建立大鼠肝细胞癌模型；检测肝细胞癌大鼠外周血中Treg细胞和Th17细胞的含量以及miR-21的表达。对miR-21表达与Treg/Th17比值之间的相关性进行统计分析。

结果

成功构建大鼠肝细胞癌模型。肝细胞癌大鼠外周血中所有CD4(+)T细胞中Th17细胞的比例为5.319%，高于对照组；而Treg细胞的比例为9.472%，高于对照组。模型组Treg/Th17比值为1.781，对照组为1.478。miR-21在肝细胞癌大鼠外周血中的表达升高，且与Treg/Th17比值呈正相关。