Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture, Sino-Dutch Joint Lab of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Hunan Vegetable Research Institute, Hunan Academy of Agricultural Science, Changsha, 410125, China.
Theor Appl Genet. 2016 Jan;129(1):97-104. doi: 10.1007/s00122-015-2612-z. Epub 2015 Oct 3.
A major QTL conditioning high degree of femaleness in cucumber was identified by marker analysis and next generation sequencing. Cucumber (Cucumis sativus L.) is a model species for sex determination studies, and its yield is associated with the degree of femaleness. Subgynoecy represents a sex form with a high degree of femaleness for which the genetic basis remains elusive. In this study, genetic analysis in the F2 and BC1 populations developed from a cross between subgynoecious S-2-98 and monoecious M95 suggested a quantitative nature of subgynoecy. Application of simple sequence repeat markers between subgynoecious and monoecious bulks constructed from BC1 plants identified three QTLs: sg3.1, sg6.1, and sg6.2. The major QTL sg3.1 contributed to 54.6% of the phenotypic variation, and its presence was confirmed by genome-wide comparison of SNP profiles between parental lines and a subgynoecious bulk constructed from BC6 plants. Using PCR-based markers developed from the SNP profile, sg3.1 was further delimited to a genomic region of 799 kb. The genetic basis of subgynoecy revealed here shall shed light on the development of elite cultivars with high yield potential.
通过标记分析和下一代测序，鉴定出一个控制黄瓜高度雌性的主要 QTL。黄瓜（Cucumis sativus L.）是性别决定研究的模式物种，其产量与雌性程度有关。亚雌株代表一种高度雌性的性别形式，其遗传基础尚不清楚。在这项研究中，来自亚雌株 S-2-98 和雌雄同株 M95 杂交的 F2 和 BC1 群体的遗传分析表明，亚雌株具有数量性状。应用来自 BC1 植物构建的亚雌株和雌雄同株群体之间的简单重复序列标记，鉴定出三个 QTL：sg3.1、sg6.1 和 sg6.2。主要 QTL sg3.1 对表型变异的贡献率为 54.6%，其存在通过对亲本系和来自 BC6 植物的亚雌株群体的 SNP 图谱进行全基因组比较得到了证实。利用从 SNP 图谱开发的基于 PCR 的标记，将 sg3.1 进一步限定在 799kb 的基因组区域内。这里揭示的亚雌株遗传基础将为开发具有高产潜力的优良品种提供启示。
