Gao Yin, Peng Jiazhu, Qiao Yanchun, Wang Guoping, Zhan Jianpo, Zhang Wensheng
Guangzhou Academy of Agricultural and Rural Sciences, Guangzhou, 510335, China.
College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
Theor Appl Genet. 2025 Apr 9;138(5):96. doi: 10.1007/s00122-025-04872-5.
It was hypothesized that Bch08G003160 (CqMYB62), located in the 51.08 Kb region on chromosome 08, might be an important candidate gene for the subgynoecy trait in chieh-qua, based on BSA-seq and linkage mapping approaches. In cucurbit crops, the use of female lines can greatly increase the yield of a single plant and is especially important for the production of hybrid generation seeds, thus being of great interest to breeders. To identify genes regulating sex differentiation in chieh-qua, genetic analysis of the subgynoecy trait was conducted using a chieh-qua F population. Initial localization of the locus was done using BSA-seq, followed by fine mapping with a large F population (n = 2,741). The locus was ultimately narrowed down to a 51.08 Kb region on Chr08, revealing a single gene Bch08G003160 (CqMYB62) in this region. Further analysis revealed that the presence of two variant loci (SNP_416 and SNP_317) in the coding region resulted in premature termination of the codon and loss of function of the structural domain of the PLN03212 superfamily. Moreover, our research indicated that the subgynoecy trait mediated by CqMYB62 in chieh-qua is potentially regulated by gibberellic acid (GA). Two efficient dCAPS markers were developed to distinguish subgynoecy. In summary, these findings highlight the critical role of CqMYB62 in subgynoecy trait regulation, offering potential implications for chieh-qua breeding programs.
基于全基因组重测序分析（BSA-seq）和连锁图谱定位方法，推测位于8号染色体上51.08 Kb区域的Bch08G003160（CqMYB62）可能是节瓜雌性弱化性状的一个重要候选基因。在葫芦科作物中，使用雌性系可以显著提高单株产量，对于杂交一代种子的生产尤为重要，因此受到育种者的广泛关注。为了鉴定调控节瓜性别分化的基因，利用节瓜F群体对雌性弱化性状进行了遗传分析。首先使用BSA-seq对该基因座进行初步定位，随后利用一个大的F群体（n = 2741）进行精细定位。最终将该基因座定位到8号染色体上一个51.08 Kb的区域，该区域有一个单一基因Bch08G003160（CqMYB62）。进一步分析发现，编码区存在两个变异位点（SNP_416和SNP_317），导致密码子提前终止，PLN03212超家族结构域功能丧失。此外，我们的研究表明，节瓜中由CqMYB62介导的雌性弱化性状可能受赤霉素（GA）调控。开发了两个有效的dCAPS标记来区分雌性弱化性状。总之，这些发现突出了CqMYB62在雌性弱化性状调控中的关键作用，为节瓜育种计划提供了潜在的参考。
