Rukdee Narisa, Rojsanga Piyanuch, Phechkrajang Chutima Matayatsuk
Nat Prod Commun. 2015 Aug;10(8):1435-7.
In this study, a triple quadrupole LC-MS/MS electrospray ionization method was developed and validated for quantitative determination of adenosine, guanosine, xanthine and uric acid in fifteen widely consumed Thai vegetables. The method was successively developed by using caffeine as internal standard. The lower limit of quantitation (LLOQ) was 0.2 μg/g for adenosine and guanosine, and 1.0 μg/g for uric acid and xanthine. The method was fully validated according to USFDA guidelines and all performance characteristics were found acceptable. Subsequently, the developed and validated LC-MS/MS method was applied to determine the four interest substances in fifteen widely consumed vegetables in Thailand. The results showed that all vegetables included in the study could be classified as low adenosine, guanosine, xanthine and uric acid containing foods since the concentrations of these substances were less than 50 mg per 100 g. This finding was enormously valuable information for hyperuricemia and gouty patients.
在本研究中，开发并验证了一种三重四极杆液相色谱-串联质谱电喷雾电离方法，用于定量测定十五种广泛食用的泰国蔬菜中的腺苷、鸟苷、黄嘌呤和尿酸。该方法以咖啡因作为内标物依次开发。腺苷和鸟苷的定量下限（LLOQ）为0.2μg/g，尿酸和黄嘌呤的定量下限为1.0μg/g。该方法根据美国食品药品监督管理局（USFDA）的指南进行了全面验证，所有性能特征均被认为是可接受的。随后，将开发并验证的液相色谱-串联质谱方法应用于测定泰国十五种广泛食用蔬菜中的四种目标物质。结果表明，该研究中包含的所有蔬菜均可归类为低腺苷、鸟苷、黄嘌呤和尿酸含量的食物，因为这些物质的浓度每100g低于50mg。这一发现对高尿酸血症和痛风患者来说是非常有价值的信息。
