Scott G K, Seow H F, Tse C A
Department of Biochemistry, University of Auckland, New Zealand.
Biochim Biophys Acta. 1989 Feb 9;1010(2):160-5. doi: 10.1016/0167-4889(89)90156-0.
Previous experiments have shown that cultured human fibroblasts possess a cell-surface proteinase (the growth-related proteinase; GRP) which is essential to cell proliferation. In the present work, proteinase inhibition in defined and complex serum-free media and in pre-conditioned normal medium, still resulted in a corresponding inhibition of cell proliferation. Proteinase inhibition also blocked the action of a range of peptide growth factors and of a phorbol ester. Elevated extracellular calcium concentrations were still mitogenic in the presence of proteinase inhibitors. Proteinase inhibition did not affect the mobilisation of intracellular calcium, nor the metabolism of inositol phosphate derivatives in response to a mitogenic stimulus.
先前的实验表明，培养的人成纤维细胞拥有一种细胞表面蛋白酶（生长相关蛋白酶；GRP），它对细胞增殖至关重要。在目前的研究中，在限定的和复杂的无血清培养基以及预处理的正常培养基中抑制蛋白酶，仍然会相应地抑制细胞增殖。蛋白酶抑制还会阻断一系列肽生长因子和佛波酯的作用。在存在蛋白酶抑制剂的情况下，细胞外钙浓度升高仍具有促有丝分裂作用。蛋白酶抑制不影响细胞内钙的动员，也不影响有丝分裂刺激下肌醇磷酸衍生物的代谢。
