Torres-Rosado A, O'Shea K S, Tsuji A, Chou S H, Kurachi K
Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109-0618.
Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7181-5. doi: 10.1073/pnas.90.15.7181.
Hepsin was previously identified as a putative cell-surface serine protease. When hepatoma cells were treated with anti-hepsin antibodies, their growth was substantially arrested, suggesting the requirement of hepsin molecules present at the cell surface for normal cell growth. This was further supported by a gross inhibition of cell growth with hepsin-specific antisense oligonucleotides. Upon treatment of cells with antisense oligonucleotides, rapid reduction in cellular hepsin was observed. This reduction in cellular hepsin levels was accompanied by drastic morphological changes. Various tissues in the developing mouse embryo showed greatly elevated hepsin levels in regions of active proliferation. These results indicate that hepsin plays an essential role in cell growth and maintenance of cell morphology.
肝胰凝乳蛋白酶先前被鉴定为一种假定的细胞表面丝氨酸蛋白酶。当用抗肝胰凝乳蛋白酶抗体处理肝癌细胞时，其生长被显著抑制，这表明细胞表面存在的肝胰凝乳蛋白酶分子是正常细胞生长所必需的。肝胰凝乳蛋白酶特异性反义寡核苷酸对细胞生长的严重抑制进一步支持了这一点。在用反义寡核苷酸处理细胞后，观察到细胞内肝胰凝乳蛋白酶迅速减少。细胞内肝胰凝乳蛋白酶水平的这种降低伴随着剧烈的形态变化。发育中的小鼠胚胎的各种组织在活跃增殖区域显示出肝胰凝乳蛋白酶水平大幅升高。这些结果表明，肝胰凝乳蛋白酶在细胞生长和细胞形态维持中起重要作用。
