Kim Myungsuk, Lim Sue Ji, Lee Hee-Ju, Nho Chu Won
Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute , Gangwon, Korea.
J Agric Food Chem. 2015 Oct 21;63(41):9037-46. doi: 10.1021/acs.jafc.5b03836. Epub 2015 Oct 13.
Cassia tora seed is widely used due to its various biological properties including anticancer, antidiabetic, and anti-inflammatory effects. However, there has been no report of the effects of C. tora seed extract (CTE) on immunoglobulin E (IgE)-mediated allergic responses. In this research, we demonstrated the effects of CTE and its active compound aurantio-obtusin on IgE-sensitized allergic reactions in mast cells and passive cutaneous anaphylaxis (PCA). CTE and aurantio-obtusin suppressed degranulation, histamine production, and reactive oxygen species generation and inhibited the production and mRNA expression of tumor necrosis factor-α and interleukin-4. CTE and aurantio-obtusin also suppressed the prostaglandin E2 production and expression of cyclooxygenase 2. Furthermore, CTE and aurantio-obtusin suppressed IgE-mediated FcεRI signaling such as phosphorylation of Syk, protein kinase Cμ, phospholipase Cγ, and extracellular signal-regulated kinases. CTE and aurantio-obtusin blocked mast cell-dependent PCA in IgE-mediated mice. These results suggest that CTE and aurantio-obtusin are a beneficial treatment for allergy-related diseases.
决明子因其多种生物学特性，包括抗癌、抗糖尿病和抗炎作用而被广泛使用。然而，尚无关于决明子种子提取物（CTE）对免疫球蛋白E（IgE）介导的过敏反应影响的报道。在本研究中，我们证明了CTE及其活性化合物橙黄决明素对肥大细胞中IgE致敏的过敏反应和被动皮肤过敏反应（PCA）的影响。CTE和橙黄决明素抑制了脱颗粒、组胺产生和活性氧生成，并抑制了肿瘤坏死因子-α和白细胞介素-4的产生及mRNA表达。CTE和橙黄决明素还抑制了前列腺素E2的产生和环氧合酶2的表达。此外，CTE和橙黄决明素抑制了IgE介导的FcεRI信号传导，如Syk、蛋白激酶Cμ、磷脂酶Cγ和细胞外信号调节激酶的磷酸化。CTE和橙黄决明素阻断了IgE介导的小鼠中肥大细胞依赖性PCA。这些结果表明，CTE和橙黄决明素是治疗过敏相关疾病的有益药物。
