Qin Shi-Han, Xu Yuan, Li Kai-Lin, Gong Kai-Yan, Peng Jie, Shi Si-Lin, Yan Fang, Cai Wei
School of Pharmacy, Weifang Medical University, Weifang 261000, China.
School of Pharmaceutical Sciences, Hunan Province Key Laboratory of Antiboby-Based Drug and Intelligent Delivery System, Hunan University of Medicine, Huaihua 418000, China.
J Anal Methods Chem. 2021 Apr 15;2021:6630604. doi: 10.1155/2021/6630604. eCollection 2021.
Aurantio-obtusin (AO) is a major anthraquinone compound isolated from or , which possesses diverse pharmacological effects. Previous studies have shown that it has a good effect on lowering blood lipids and treating various diseases. A few studies have also reported about its metabolites. A rapid and reliable method using ultra-high-performance liquid chromatography-Q-Exactive Orbitrap mass spectrometry and multiple data-processing technologies was established to investigate the metabolites of AO in the plasma and various tissues of rats, including the heart, liver, spleen, lung, kidneys, and brain. Finally, a total of 36 metabolites were identified in the plasma of rats, which could be very beneficial for understanding the effective form of AO metabolites leading to new drug discovery. The result demonstrated that this strategy, especially parallel reaction monitoring, has shown a wide range of applications in the identification of metabolites.
橙黄决明素（AO）是从[具体植物名称1]或[具体植物名称2]中分离出的一种主要蒽醌化合物，具有多种药理作用。先前的研究表明，它在降低血脂和治疗各种疾病方面有良好效果。也有一些研究报道了其代谢产物。建立了一种使用超高效液相色谱-Q-Exactive Orbitrap质谱联用技术和多种数据处理技术的快速可靠方法，用于研究AO在大鼠血浆及心脏、肝脏、脾脏、肺、肾脏和大脑等各种组织中的代谢产物。最终，在大鼠血浆中总共鉴定出36种代谢产物，这对于理解AO代谢产物的有效形式以推动新药研发可能非常有益。结果表明，该策略，尤其是平行反应监测，在代谢产物鉴定方面已显示出广泛的应用。
