Pratedrat Pornpitra, Sopipong Watanyoo, Makkoch Jarika, Praianantathavorn Kesmanee, Chuaypen Natthaya, Tangkijvanich Pisit, Payungporn Sunchai
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Thailand E-mail :
Asian Pac J Cancer Prev. 2015;16(15):6457-61. doi: 10.7314/apjcp.2015.16.15.6457.
MicroRNAs directly and indirectly influence many biological processes such as apoptosis, cell maintenance, and immune responses, impacting on tumor genesis and metastasis. They modulate gene expression at the post- transcriptional level and are associated with progression of liver disease. Hepatocellular carcinoma (HCC) is a cancer which mostly occurs in males. There are many factors affect HCC development, for example, hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV), co-infection, environmental factors including alcohol, aflatoxin consumption and host-related factors such as age, gender immune response, microRNA and single nucleotide polymorphisms (SNPs). Chronic infection with the hepatitis B virus is the major factor leading to HCC progression since it causes the liver injury. At present, there are many reports regarding the association of SNPs on miRNAs and the HCC progression. In this research, we investigated the role of miR- 149 (rs2292832) and miR-101-1 (rs7536540) with HCC progression in Thai population. The study included 289 Thai subjects including 104 HCC patients, 90 patients with chronic hepatitis B virus infection (CHB) and 95 healthy control subjects. The allele and genotype of rs2292832 and rs7536540 polymorphisms were determined by TaqMan real-time PCR assay. Our results revealed no significant association between miR-149 (rs2292832) and miR-101-1 (rs7536540) and the risk of HCC in our Thai population. However, this research is the first study of miR-149 (rs2292832) and miR-101-1 (rs7536540) in HCC in Thai populations and the results need to be confirmed with a larger population.
微小RNA直接或间接影响许多生物学过程，如细胞凋亡、细胞维持和免疫反应，进而影响肿瘤的发生和转移。它们在转录后水平调节基因表达，并与肝脏疾病的进展相关。肝细胞癌（HCC）是一种主要发生在男性中的癌症。有许多因素影响HCC的发展，例如，乙型肝炎病毒（HBV）、丙型肝炎病毒（HCV）和人类免疫缺陷病毒（HIV）、合并感染、包括酒精、黄曲霉毒素摄入等环境因素以及宿主相关因素，如年龄、性别、免疫反应、微小RNA和单核苷酸多态性（SNP）。慢性乙型肝炎病毒感染是导致HCC进展的主要因素，因为它会引起肝损伤。目前，有许多关于SNP与微小RNA及HCC进展相关性的报道。在本研究中，我们调查了miR-149（rs2292832）和miR-101-1（rs7536540）在泰国人群HCC进展中的作用。该研究纳入了289名泰国受试者，包括104例HCC患者、90例慢性乙型肝炎病毒感染（CHB）患者和95名健康对照者。通过TaqMan实时PCR检测法确定rs2292832和rs7536540多态性的等位基因和基因型。我们的结果显示，在我们的泰国人群中，miR-149（rs2292832）和miR-101-1（rs7536540）与HCC风险之间无显著关联。然而，本研究是首次在泰国人群中对miR-149（rs2292832）和miR-101-1（rs7536540）进行的HCC研究，其结果需要在更大规模人群中得到证实。
