Sarto F, Zordan M, Tomanin R, Mazzotti D, Canova A, Cardin E L, Bezze G, Levis A G
Institute of Occupational Health, Padova, Italy.
Carcinogenesis. 1989 Feb;10(2):329-34. doi: 10.1093/carcin/10.2.329.
Six male non-smoking subjects treated for psoriasis with topical applications of pure coal tar or 4% coal tar-containing ointment were examined in order to assess the genotoxic risk associated with this type of therapy. Mutagenicity in urine samples collected before and during the coal tar therapy was evaluated in the plate incorporation assay on Salmonella typhimurium strain TA98 in the presence of S9 mix and beta-glucuronidase. Total urinary polycyclic aromatic hydrocarbon (PAH) levels were evaluated in parallel by high resolution gas chromatography/mass spectrometry. In addition, sister chromatid exchanges and chromosomal aberrations were also analysed in peripheral blood lymphocytes collected before, during and after the end of the coal tar applications. The results suggest that urinary mutagenicity levels as well as the frequencies of chromosome aberrations and sister chromatid exchanges in lymphocytes are related to the levels of exposure to coal tar. Moreover the kinetics of repair of chromosome damage in relation to different exposure levels and the capacity of the urinary mutagenicity assay to correctly identify the exposure to significant levels of PAH are discussed.
为了评估与这种治疗方式相关的基因毒性风险，对6名接受纯煤焦油或含4%煤焦油软膏局部治疗银屑病的男性非吸烟受试者进行了检查。在存在S9混合物和β-葡萄糖醛酸酶的情况下，采用鼠伤寒沙门氏菌TA98菌株平板掺入试验，评估煤焦油治疗前和治疗期间采集的尿液样本的致突变性。通过高分辨率气相色谱/质谱法同时评估尿液中多环芳烃（PAH）的总水平。此外，还对煤焦油涂抹前、涂抹期间和涂抹结束后采集的外周血淋巴细胞中的姐妹染色单体交换和染色体畸变进行了分析。结果表明，尿液致突变性水平以及淋巴细胞中染色体畸变和姐妹染色单体交换的频率与煤焦油暴露水平有关。此外，还讨论了与不同暴露水平相关的染色体损伤修复动力学以及尿液致突变性检测正确识别显著水平PAH暴露的能力。
