Azimi Ako, Hagh Majid Farshdousti, Talebi Mehdi, Yousefi Bahman, Hossein pour feizi Abbas Ali, Baradaran Behzad, Movassaghpour Ali Akbar, Shamsasenjan Karim, Khanzedeh Taghi, Ghaderi Abdol Hasan, Heydarabad Milad Zadi
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran E-mail :
Asian Pac J Cancer Prev. 2015;16(15):6463-8. doi: 10.7314/apjcp.2015.16.15.6463.
Chemotherapy is one of the common approaches in treatment of cancers, especially leukemia. However, drug resistance phenomena reduce the likelihood of treatment success. Resveratrol is a herbal compound which through complicated processes makes some selected cells sensitive to treatment and induction of apoptosis. In the present study, the effects of resveratrol on the expression of miR 15a and miR16-1 and apoptosis in the CCRF-CEM cell line were investigated.
The CCRF-CEM cell line was cultured under standard conditions and changes in miR 15a and miR 16-1 expression were analyzed by real time-PCR technique, with attention to reveratrol dose and time dependence. Also, apoptosis is evaluated by flow cytometry using annexin V and PI.
CCRF-CEM cells underwent dose-dependent apoptotic cell death in response to resveratrol. MiR 15a and miR 16-1 expression was up-regulated after 24 and 48 hours resveratrol treatment (p<0.05).
The results of our study indicate that resveratrol induces apoptosis in a time and dose- dependent manner in CCRF-CEM cells. Also, increased expression level of miR 16-1 and miR 15a by means of resveratrol in CCRF-CEM cells might have a role in apoptosis induction and predisposition. According to our results resveratrol can be regarded as a dietary supplement to improve efficacy of anti-leukemia therapies.
化疗是治疗癌症尤其是白血病的常见方法之一。然而，耐药现象降低了治疗成功的可能性。白藜芦醇是一种植物化合物，它通过复杂的过程使某些特定细胞对治疗敏感并诱导细胞凋亡。在本研究中，研究了白藜芦醇对CCRF - CEM细胞系中miR 15a和miR16 - 1表达及细胞凋亡的影响。
CCRF - CEM细胞系在标准条件下培养，采用实时PCR技术分析miR 15a和miR 16 - 1表达的变化，并关注白藜芦醇剂量和时间依赖性。此外，使用膜联蛋白V和碘化丙啶通过流式细胞术评估细胞凋亡。
CCRF - CEM细胞对白藜芦醇呈剂量依赖性凋亡细胞死亡。白藜芦醇处理24小时和48小时后，miR 15a和miR 16 - 1表达上调（p<0.05）。
我们的研究结果表明，白藜芦醇在CCRF - CEM细胞中以时间和剂量依赖性方式诱导细胞凋亡。此外，白藜芦醇使CCRF - CEM细胞中miR 16 - 1和miR 15a表达水平升高可能在诱导细胞凋亡和易感性方面发挥作用。根据我们的结果，白藜芦醇可被视为一种膳食补充剂，以提高抗白血病治疗的疗效。
