Yuan W, Hua R, Zhang X, Sun M, Zhang L, Chen L, Sun J
Acta Virol. 2015 Sep;59(3):300-4. doi: 10.4149/av_2015_03_300.
Encephalomyocarditis virus (EMCV) can cause acute myocarditis in young pigs or reproductive failure in sows. The BD2 strain was isolated from the suspected piglets with EMCV in China. In order to establish an experimental animal model of EMCV, eight-weeks-old male BALB/c mice were intraperitonealy inoculated with 0.1 ml of 4×10(5) TCID50 suspension of the EMCV. Infected mice demonstrated hind limb paralysis, and movement disorder. The mortality rate of the infected group was 100% during the one-week observation period. The viral load in the brain of challenged mice gradually increased, with a peak level being 6.53 log CCID50/0.1 ml 5 days post infection. The pathological injury in infected mice was presented as neuronal necrosis. Brown positive staining could be detected in the cytoplasm of cerebral neurons. These results indicate that the porcine EMCV isolated from China could replicate in brain tissues and induce acute encephalitis in BALB/c mice.
脑心肌炎病毒（EMCV）可导致幼猪急性心肌炎或母猪繁殖失败。BD2毒株是从中国疑似感染EMCV的仔猪中分离出来的。为建立EMCV实验动物模型，将0.1 ml含4×10(5) TCID50的EMCV悬液腹腔注射到8周龄雄性BALB/c小鼠体内。感染小鼠出现后肢麻痹和运动障碍。在为期一周的观察期内，感染组的死亡率为100%。攻毒小鼠脑内病毒载量逐渐升高，感染后5天达到峰值水平6.53 log CCID50/0.1 ml。感染小鼠的病理损伤表现为神经元坏死。在脑神经元细胞质中可检测到棕色阳性染色。这些结果表明，从中国分离的猪源EMCV可在脑组织中复制并诱导BALB/c小鼠发生急性脑炎。
