Department of Bioengineering and Aerospace Engineering, Universidad Carlos III de Madrid, 28911 Madrid, Spain; Experimental Medicine and Surgery Unit, Instituto de Investigación Sanitaria Gregorio Marañón, 28007 Madrid, Spain.
Carl Zeiss AG, Corporate Research and Technology, Carl-Zeiss-Promenade 10, 07745 Jena, Germany; Institute of Biological and Medical Imaging (IBMI), Technische Universität München and Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
Trends Biotechnol. 2015 Nov;33(11):679-691. doi: 10.1016/j.tibtech.2015.08.002. Epub 2015 Oct 1.
The past decade marked an optical revolution in biology: an unprecedented number of optical techniques were developed and adopted for biological exploration, demonstrating increasing interest in optical imaging and in vivo interrogations. Optical methods have become faster and have reached nanoscale resolution, and are now complemented by optoacoustic (photoacoustic) methods capable of imaging whole specimens in vivo. Never before were so many optical imaging barriers broken in such a short time-frame: with new approaches to optical microscopy and mesoscopy came an increased ability to image biology at unprecedented speed, resolution, and depth. This review covers the most relevant techniques for imaging in developmental biology, and offers an outlook on the next steps for these technologies and their applications.
空前数量的光学技术被开发并应用于生物探索，这表明人们对光学成像和活体检测越来越感兴趣。光学方法变得更快，达到了纳米级分辨率，现在又有了光声（超声）方法来补充，能够对整个活体标本进行成像。在如此短的时间内，如此多的光学成像障碍被打破：通过新的光学显微镜和介观显微镜方法，人们以前所未有的速度、分辨率和深度来研究生物学的能力得到了增强。本综述涵盖了发育生物学中最相关的成像技术，并对这些技术及其应用的下一步发展进行了展望。
