Guo Tianyang, Liu Yuanbo, Wei Yanxiu, Ma Xiang, Fan Qiuming, Ni Jun, Yin Zequn, Liu Jiahui, Wang Sai, Dong Yiyang, Zhang Jichuan, Zhang Liqun, Su Haijia, Tan Tianwei
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Center of Advanced Elastomer Materials, College of Materials and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Nov 1;1004:17-22. doi: 10.1016/j.jchromb.2015.09.007. Epub 2015 Sep 18.
Natural trans-1,4-polyisoprene (TPI) as a functional biomaterial has aroused great interest for rubber industrial product use. Here, we proposed a method that enables simultaneous analysis of the content and molecular-weight distribution (MWD) of natural TPI by gel permeation chromatography (GPC). The natural TPIs were collected from leaves, fruit coatings and bark of Eucommia ulmoides Oliver (E. ulmoides) through toluene extraction followed by ethanol purification. The results of TPI contents from leaves and fruit coatings were shown ca. 3.5% and 13.8%, respectively. Accordingly, limits of detection (LODs) of TPI were 0.58mg/mL from leaves and 0.47mg/mL from fruit coatings. The MWDs of TPI demonstrated a bimodal distribution from leaves, a unimodal distribution from bark, and a unimodal distribution with a tiny peak shoulder from fruit coatings. In real-life E. ulmoides analysis, the results from three independent methods (GPC, gravimetric method, and infrared spectroscopy) were obtained with good consistency.
天然反式-1,4-聚异戊二烯（TPI）作为一种功能性生物材料，已引起橡胶工业产品应用领域的极大兴趣。在此，我们提出了一种通过凝胶渗透色谱法（GPC）同时分析天然TPI含量和分子量分布（MWD）的方法。通过甲苯萃取，然后乙醇纯化，从杜仲（Eucommia ulmoides Oliver，E. ulmoides）的叶子、果实外皮和树皮中收集天然TPI。叶子和果实外皮中TPI含量的结果分别约为3.5%和13.8%。相应地，叶子中TPI的检测限（LOD）为0.58mg/mL，果实外皮中为0.47mg/mL。TPI的MWD在叶子中呈双峰分布，在树皮中呈单峰分布，在果实外皮中呈带有微小峰肩的单峰分布。在实际杜仲分析中，三种独立方法（GPC、重量法和红外光谱法）的结果具有良好的一致性。
