Rai Mahendra, Ingle Avinash P, Gade Aniket K, Duarte Marta Cristina Teixeira, Duran Nelson
Biological Chemistry Laboratory, Institute of Chemistry, University of Campinas, Campinas, SP, Brazil.
Department of Biotechnology, Nanobiotechnology Laboratory, SGB Amravati University, Amravati 444602, Maharashtra, India.
IET Nanobiotechnol. 2015 Oct;9(5):280-7. doi: 10.1049/iet-nbt.2014.0068.
The authors report extracellular mycosynthesis of silver nanoparticles (AgNPs) by Phoma capsulatum, Phoma putaminum and Phoma citri. The AgNPs thus synthesised were characterised by UV-visible spectrophotometer, Fourier transform infrared spectroscopy, Nanosight LM20 and transmission electron microscopy, which confirmed the synthesis of mostly spherical and polydisperse nanoparticles capped with proteins. The size of AgNPs was found in the range of 10-80 , 5-80 and 5-90 nm with an average size of 31.85, 25.43 and 23.29 nm by P. capsulatum, P. putaminum and P. citri, respectively. Further, potential antimicrobial activity was reported against Aspergillus niger, Candida albicans, Salmonella choleraesuis, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The lowest minimal inhibitory concentration (MIC) (0.85 µg/ml) was reported for AgNPs synthesised from P. citri against S. choleraesuis. However, AgNPs synthesised from P. capsulatum showed the highest MIC (10.62 µg/ml) against S. choleraesuis, P. aeruginosa and E. coli (clinical isolate). The same MIC values (10.62 µg/ml) were also reported against P. aeruginosa and both clinical and standard isolates of E. coli for AgNPs synthesised from P. citri. It was also observed that all the silver nanoparticles showed remarkable antifungal and antibacterial activity against these tested pathogens as compared with the commercially available antifungal and antibacterial agents.
作者报告了荚膜茎点霉、腐烂茎点霉和柑橘茎点霉对银纳米颗粒（AgNPs）的胞外真菌合成。通过紫外可见分光光度计、傅里叶变换红外光谱、纳米视界LM20和透射电子显微镜对由此合成的AgNPs进行了表征，证实合成的主要是由蛋白质包覆的球形且多分散的纳米颗粒。荚膜茎点霉、腐烂茎点霉和柑橘茎点霉合成的AgNPs尺寸分别在10 - 80、5 - 80和5 - 90 nm范围内，平均尺寸分别为31.85、25.43和23.29 nm。此外，报告了其对黑曲霉、白色念珠菌、猪霍乱沙门氏菌、铜绿假单胞菌、金黄色葡萄球菌和大肠杆菌具有潜在抗菌活性。柑橘茎点霉合成的AgNPs对猪霍乱沙门氏菌的最低最小抑菌浓度（MIC）为0.85 μg/ml。然而，荚膜茎点霉合成的AgNPs对猪霍乱沙门氏菌、铜绿假单胞菌和大肠杆菌（临床分离株）的MIC最高，为10.62 μg/ml。柑橘茎点霉合成的AgNPs对铜绿假单胞菌以及大肠杆菌的临床和标准分离株的MIC值也为10.62 μg/ml。还观察到，与市售抗真菌和抗菌剂相比，所有银纳米颗粒对这些测试病原体均表现出显著的抗真菌和抗菌活性。
