Chatterjee D, Bozic C M, Knisley C, Cho S N, Brennan P J
Department of Microbiology, Colorado State University, Fort Collins 80523.
Infect Immun. 1989 Feb;57(2):322-30. doi: 10.1128/iai.57.2.322-330.1989.
The glycolipid that characterizes the majority of isolates of Mycobacterium bovis and that has come to be known as M. bovis-identifying lipid is the phenolic glycolipid mycoside B described in the literature by others. However, when mycoside B obtained from M. bovis BCG, field isolates, and infected tissues was examined in detail, it was shown to be different from that described in the literature in some important respects. In particular, the glycosyl substituent is 2-O-methyl-alpha-L-rhamnopyranose rather than 2-O-methyl-beta-D-rhamnopyranose. With this information, a seroreactive neoglycoprotein (neoantigen) containing the 2-O-methyl-alpha-L-rhamnopyranosyl substituent suitable for the serodiagnosis of bovine tuberculosis was synthesized. M. bovis also contains other minor seroreactive phenolic glycolipids, one of which is a deacylated form of mycoside B and another of which contains an alpha-L-rhamnopyranosyl unit rather than 2-O-methyl-alpha-L-rhamnopyranose.
牛分枝杆菌大多数菌株所特有的、已被称为牛分枝杆菌鉴定性脂质的糖脂是其他人在文献中描述的酚糖脂霉菌糖苷B。然而，当对从卡介苗、野外分离株和感染组织中获得的霉菌糖苷B进行详细检测时，发现它在一些重要方面与文献中描述的不同。特别是，糖基取代基是2-O-甲基-α-L-鼠李吡喃糖，而不是2-O-甲基-β-D-鼠李吡喃糖。基于这一信息，合成了一种含有适合牛结核病血清诊断的2-O-甲基-α-L-鼠李吡喃糖基取代基的血清反应性新糖蛋白（新抗原）。牛分枝杆菌还含有其他次要的血清反应性酚糖脂，其中一种是霉菌糖苷B的脱酰基形式，另一种含有α-L-鼠李吡喃糖基单元而不是2-O-甲基-α-L-鼠李吡喃糖。
