Barrow W W, Brennan P J
Infect Immun. 1982 May;36(2):678-84. doi: 10.1128/iai.36.2.678-684.1982.
Specific rabbit antibodies were obtained from the inoculation of a complex composed of methylated bovine serum albumin and the superficial type-specific C-mycoside glycopeptidolipid (GPL) antigens from serovar 20 of the Mycobacterium avium-M. intracellulare-M. scrofulaceum complex. Although the GPLs on their own are serologically active, they require the protein carrier for immunogenicity. Antibodies to the complex were detected by seroagglutination, gel diffusion, and indirect immunofluorescence. Cross-reaction was observed in the case of just one of 16 M. avium-M. intracellulare-M. scrofulaceum complex serovars tested (serovar 41). Double gel diffusion and immunoelectrophoresis showed two major antigen-antibody complexes in the reaction between anti-GPL-methylated bovine serum albumin and the GPL antigens. The studies of migration pattern and absorption indicated that immunoglobulin M was responsible for one of these complexes, whereas the antibody responsible for the second complex was unidentified. The specific anti-GPL serum is a useful tool for the identification and classification of atypical mycobacteria and for the study of the fate of the organisms and their superficies during phagocytosis.
通过接种由甲基化牛血清白蛋白和鸟分枝杆菌-胞内分枝杆菌-瘰疬分枝杆菌复合体血清型20的浅表型特异性C-霉菌糖苷糖脂（GPL）抗原组成的复合物，获得了特异性兔抗体。尽管GPL自身具有血清学活性，但它们需要蛋白质载体来产生免疫原性。通过血清凝集、凝胶扩散和间接免疫荧光检测针对该复合物的抗体。在所检测的16种鸟分枝杆菌-胞内分枝杆菌-瘰疬分枝杆菌复合体血清型中，仅在一种血清型（血清型41）中观察到交叉反应。双向凝胶扩散和免疫电泳显示，在抗GPL-甲基化牛血清白蛋白与GPL抗原的反应中有两种主要的抗原-抗体复合物。迁移模式和吸收研究表明，免疫球蛋白M负责其中一种复合物，而负责第二种复合物的抗体尚未确定。特异性抗GPL血清是用于非典型分枝杆菌鉴定和分类以及研究吞噬过程中生物体及其表面命运的有用工具。
