用于尿液培养筛查的BACTEC系统评估
Evaluation of BACTEC system for urine culture screening.
作者信息
Colombrita D, Ravizzola G, Pirali F, Manni M, Manca N, Savoldi E, Turano A
机构信息
Institute of Microbiology, Brescia University, Italy.
出版信息
J Clin Microbiol. 1989 Jan;27(1):118-9. doi: 10.1128/jcm.27.1.118-119.1989.
We compared urine culturing performed by the calibrated loop method with a screening system (BACTEC). A total of 852 urine specimens were examined by both the conventional loop method and the BACTEC system. With the loop method, 193 (22.6%) urine samples were positive, whereas with the BACTEC system, 185 (21.7%) were positive (sensitivity, 96.01%). At a breakpoint of 10(4) CFU/ml, eight false-negatives were detected (sensitivity, 87.09%), and at a breakpoint of 10(5) CFU/ml, four false-negatives were observed (sensitivity, 97.6%). The specificity of the BACTEC system was 100%. We propose the BACTEC method as an effective alternative to the other growth-dependent screening tests for bacteriuria.
我们将校准定量环法进行的尿液培养与一种筛查系统（BACTEC）进行了比较。总共852份尿液标本通过传统定量环法和BACTEC系统进行了检测。采用定量环法时，193份（22.6%）尿液样本呈阳性，而采用BACTEC系统时，185份（21.7%）呈阳性（敏感性为96.01%）。在10⁴CFU/ml的分界点时，检测到8例假阴性（敏感性为87.09%），在10⁵CFU/ml的分界点时，观察到4例假阴性（敏感性为97.6%）。BACTEC系统的特异性为100%。我们建议将BACTEC方法作为其他依赖细菌生长的菌尿筛查试验的有效替代方法。
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