Wang Wei, Gao Li, Li Yan, Li Zhen-Ling, Gong Ming, Huang Fan-Zhou, Chen Yan-Rong, Zhang Chun-Xia, Gao Ya-Yue, Ma Yi-Gai
a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
Leuk Lymphoma. 2016 May;57(5):1174-81. doi: 10.3109/10428194.2015.1070153. Epub 2015 Oct 5.
The expression of CD73 by flow cytometry (FC) in bone marrow (BM) specimens of B-cell acute lymphoblastic leukemia (B-ALL) with or without minimal residual disease (MRD) was studied, and its advantages were evaluated using the MRD assay. This study also detected the expression profile of CD73 in hematogones and mature B cells in BM specimens of 18 healthy donors. Results showed that the mean value of CD73 expression in MRD-positive B cells was 6-fold greater than that in the MRD negative ones. Also, 41.82% MRD-positive B-ALL cases expressed high CD73 and the sensitivity of CD73-based MRD detection reached 10(-4). Since the expression of CD73 increases with the maturation of normal B cells, it is better to mix it with CD34, CD10 and CD20 in one tube to prevent the disturbance of mature B cells. CD73 is recommended as an optional MRD marker for B-ALL patients by using FC.
研究了通过流式细胞术（FC）检测有或无微小残留病（MRD）的B细胞急性淋巴细胞白血病（B-ALL）骨髓（BM）标本中CD73的表达，并使用MRD检测评估其优势。本研究还检测了18名健康供者BM标本中造血细胞和成熟B细胞中CD73的表达谱。结果显示，MRD阳性B细胞中CD73表达的平均值比MRD阴性B细胞高6倍。此外，41.82%的MRD阳性B-ALL病例表达高水平的CD73，基于CD73的MRD检测灵敏度达到10（-4）。由于CD73的表达随着正常B细胞的成熟而增加，最好将其与CD34、CD10和CD20在一管中混合，以防止成熟B细胞的干扰。建议通过FC将CD73作为B-ALL患者的一种可选MRD标志物。
