菌株ŽP - 首个基于细菌接合作用的“杀伤”-“抗杀伤”抗菌系统。

Strain ŽP - the first bacterial conjugation-based "kill"-"anti-kill" antimicrobial system.

作者信息

Starčič Erjavec Marjanca, Petkovšek Živa, Kuznetsova Marina V, Maslennikova Irina L, Žgur-Bertok Darja

机构信息

Department of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.

Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Science, 13 Golev Street, 614081 Perm, Russia.

出版信息

Plasmid. 2015 Nov;82:28-34. doi: 10.1016/j.plasmid.2015.10.001. Epub 2015 Oct 3.

DOI:10.1016/j.plasmid.2015.10.001

PMID:26436830

Abstract

As multidrug resistant bacteria pose one of the greatest risks to human health new alternative antibacterial agents are urgently needed. One possible mechanism that can be used as an alternative to traditional antibiotic therapy is transfer of killing agents via conjugation. Our work was aimed at providing a proof of principle that conjugation-based antimicrobial systems are possible. We constructed a bacterial conjugation-based "kill"-"anti-kill" antimicrobial system employing the well known Escherichia coli probiotic strain Nissle 1917 genetically modified to harbor a conjugative plasmid carrying the "kill" gene (colicin ColE7 activity gene) and a chromosomally encoded "anti-kill" gene (ColE7 immunity gene). The constructed strain acts as a donor in conjugal transfer and its efficiency was tested in several types of conjugal assays. Our results clearly demonstrate that conjugation-based antimicrobial systems can be highly efficient.

摘要

由于多重耐药细菌对人类健康构成了最大风险之一，因此迫切需要新的替代抗菌剂。一种可作为传统抗生素治疗替代方法的可能机制是通过接合转移杀伤剂。我们的工作旨在证明基于接合的抗菌系统是可行的。我们构建了一种基于细菌接合的“杀伤”-“抗杀伤”抗菌系统，该系统使用了著名的大肠杆菌益生菌菌株Nissle 1917，对其进行基因改造，使其携带一个携带“杀伤”基因（大肠杆菌素ColE7活性基因）的接合质粒和一个染色体编码的“抗杀伤”基因（ColE7免疫基因）。构建的菌株在接合转移中作为供体，其效率在几种类型的接合试验中进行了测试。我们的结果清楚地表明，基于接合的抗菌系统可以非常高效。