一种通过比色法测定肠球菌和葡萄球菌对抗生素敏感性的两小时检测方法。
A two-hour procedure for determining the susceptibility of enterococci and staphylococci to antibiotics by a colourimetric method.
作者信息
March-Rosselló Gabriel Alberto, Gutiérrez-Rodríguez María Purificación, Simarro-Grande María, Orduña-Domingo Antonio, Bratos-Pérez Miguel Angel
机构信息
Gabriel Alberto March Rosselló, Servicio de Microbiología e Inmunología, Hospital Clínico Universitario de Valladolid, Spain.
出版信息
Rev Esp Quimioter. 2015 Oct;28(5):247-55.
INTRODUCTION
Rapid determination of the antibiotic susceptibility test in bacteria remains a challenge for Clinical Microbiology laboratories.
METHODS
An improvement in the colorimetric antimicrobial susceptibility testing performed with resazurin in enterococci and staphylococci has been carried out. The design of method was performed using two collection strains, which have a known susceptibility. This procedure was then validated against standard commercial methods on 15 strains of staphylococci and 15 strains of enterococci from patients.
RESULTS
The essential agreement between the colorimetric method and commercial methods (E-test, MicroScan and VITEK2) was 100%.
CONCLUSION
Resazurin allows us to obtain a reliable antibiotic susceptibility test in staphylococci and enterococci in less than two hours.
引言
快速确定细菌中的抗生素敏感性试验仍是临床微生物实验室面临的一项挑战。
方法
已对使用刃天青对肠球菌和葡萄球菌进行的比色抗菌药物敏感性试验进行了改进。该方法的设计使用了两种已知敏感性的收集菌株。然后针对来自患者的15株葡萄球菌和15株肠球菌，根据标准商业方法对该程序进行了验证。
结果
比色法与商业方法（E-test、MicroScan和VITEK2）之间的基本一致性为100%。
结论
刃天青使我们能够在不到两小时的时间内获得可靠的葡萄球菌和肠球菌抗生素敏感性试验结果。
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