通过ATP生物发光法进行的两小时抗生素敏感性测试。

A two-hour antibiotic susceptibility test by ATP-bioluminescence.

作者信息

March Rosselló Gabriel Alberto, García-Loygorri Jordán de Urries María Cristina, Gutiérrez Rodríguez María Purificación, Simarro Grande María, Orduña Domingo Antonio, Bratos Pérez Miguel Ángel

机构信息

Departamento de Microbiología, Facultad de Medicina, Universidad de Valladolid, Spain.

Servicio de Microbioología y Parasitología, Hospital de Medina del Campo, Spain.

出版信息

Enferm Infecc Microbiol Clin. 2016 Jun-Jul;34(6):334-9. doi: 10.1016/j.eimc.2015.03.013. Epub 2015 May 12.

DOI:10.1016/j.eimc.2015.03.013

PMID:25979598

Abstract

The antibiotic susceptibility test (AST) in Clinical Microbiology laboratories is still time-consuming, and most procedures take 24h to yield results. In this study, a rapid antimicrobial susceptibility test using ATP-bioluminescence has been developed. The design of method was performed using five ATCC collection strains of known susceptibility. This procedure was then validated against standard commercial methods on 10 strains of enterococci, 10 staphylococci, 10 non-fermenting gram negative bacilli, and 13 Enterobacteriaceae from patients. The agreement obtained in the sensitivity between the ATP-bioluminescence method and commercial methods (E-test, MicroScan and VITEK2) was 100%. In summary, the preliminary results obtained in this work show that the ATP-bioluminescence method could provide a fast and reliable AST in two hours.

摘要

临床微生物实验室中的抗生素敏感性试验（AST）仍然耗时，大多数检测程序需要24小时才能得出结果。在本研究中，已开发出一种使用ATP生物发光的快速抗菌药敏试验。该方法的设计使用了五种已知药敏性的ATCC收集菌株。然后，针对来自患者的10株肠球菌、10株葡萄球菌、10株非发酵革兰氏阴性杆菌和13株肠杆菌科细菌，根据标准商业方法对该程序进行了验证。ATP生物发光法与商业方法（E-test、MicroScan和VITEK2）在敏感性方面的一致性为100%。总之，这项工作获得的初步结果表明，ATP生物发光法可以在两小时内提供快速可靠的AST。