Hoseinifar Seyed Hossein, Khalili Mohsen, Rufchaei Rudabeh, Raeisi Mojtaba, Attar Marzieh, Cordero Héctor, Esteban M Ángeles
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Medical Cellular & Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Fish Shellfish Immunol. 2015 Dec;47(2):706-11. doi: 10.1016/j.fsi.2015.09.046. Epub 2015 Oct 9.
The aim of this study was to investigate the effects of date palm fruit extracts (DPFE) on skin mucosal immunity, immune related genes expression and growth performance of fry common carp (Cyprinus carpio). One hundred and twenty specimens (4.06 ± 0.13 g) were supplied and allocated into six aquaria; specimens in three aquaria were fed non-supplemented diet (control) while the fish in the other 3 aquaria were fed with DPFE at 200 ml kg(-1). At the end of feeding trial (8 weeks) skin mucus immune parameters (total immunoglobulins, lysozyme, protease and alkaline phosphatase activity) and immune related gene expression (tumor necrosis factor α [tnfa], lysozyme [ly] and interleukin-1-beta, [il1b]) in the head-kidney were studied. The results revealed that feeding carp fry with 200 ml kg(-1) DPFE remarkably elevated the three skin mucus immune parameters tested (P < 0.05). However, evaluation of immune related gene expression demonstrated that the expression of tnfa and il1b was considerably decreased (P < 0.05) in fish fed DPFE diet, while the expression of ly remained similar (P > 0.05) compared to control fish (fed control diet). Furthermore, growth performance parameters were significantly improved in fry fed DPFE (P < 0.05). More studies are needed to understand different aspects of DPFE administration in fry mucosal immunity.
本研究的目的是探讨海枣果实提取物（DPFE）对鲤鱼幼鱼皮肤黏膜免疫、免疫相关基因表达及生长性能的影响。共提供120尾样本（4.06±0.13克），并将其分配到六个水族箱中；三个水族箱中的样本投喂未添加提取物的饲料（对照），而另外三个水族箱中的鱼则投喂200毫升/千克的DPFE。在饲养试验结束时（8周），研究了皮肤黏液免疫参数（总免疫球蛋白、溶菌酶、蛋白酶和碱性磷酸酶活性）以及头肾中免疫相关基因的表达（肿瘤坏死因子α[tnfa]、溶菌酶[ly]和白细胞介素-1β[il1b]）。结果显示，用200毫升/千克的DPFE投喂鲤鱼幼鱼显著提高了所检测的三项皮肤黏液免疫参数（P<0.05）。然而，免疫相关基因表达评估表明，与对照鱼（投喂对照饲料）相比，投喂DPFE饲料的鱼中tnfa和il1b的表达显著降低（P<0.05），而ly的表达保持相似（P>0.05）。此外，投喂DPFE的幼鱼生长性能参数显著提高（P<0.05）。需要进行更多研究以了解DPFE在幼鱼黏膜免疫中的不同作用方面。
