O'Donnell Hope, Pham Oanh H, Benoun Joseph M, Ravesloot-Chávez Marietta M, McSorley Stephen J
Center for Comparative Medicine, Department of Anatomy, Physiology & Cell Biology, University of California Davis, Davis, CA, USA.
Yersinia Research Unit, Microbiology Department, Pasteur Institute, Paris, France.
Future Microbiol. 2015;10(10):1615-27. doi: 10.2217/fmb.15.93. Epub 2015 Oct 6.
In most infectious disease models, it is assumed that gavage needle infection is the most reliable means of pathogen delivery to the GI tract. However, this methodology can cause esophageal tearing and induces stress in experimental animals, both of which have the potential to impact early infection and the subsequent immune response.
MATERIALS & METHODS: C57BL/6 mice were orally infected with virulent Salmonella Typhimurium SL1344 either by intragastric gavage preceded by sodium bicarbonate, or by contamination of drinking water.
We demonstrate that water contamination delivery of Salmonella is equivalent to gavage inoculation in providing a consistent model of infection. Furthermore, exposure of mice to contaminated drinking water for as little as 4 h allowed maximal mucosal and systemic infection, suggesting an abbreviated window exists for natural intestinal entry.
Together, these data question the need for gavage delivery for infection with oral pathogens.
在大多数传染病模型中，假定灌胃针感染是将病原体递送至胃肠道最可靠的方式。然而，这种方法可能导致食管撕裂并在实验动物中诱发应激，这两者都有可能影响早期感染及随后的免疫反应。
C57BL/6小鼠通过先给予碳酸氢钠然后进行灌胃的方式，或通过饮用污染水的方式口服感染强毒鼠伤寒沙门氏菌SL1344。
我们证明，沙门氏菌通过水污染递送在提供一致的感染模型方面等同于灌胃接种。此外，小鼠暴露于污染饮用水仅4小时即可实现最大程度的黏膜和全身感染，这表明存在一个短暂的自然肠道进入窗口期。
总之，这些数据对使用灌胃方式递送口服病原体进行感染的必要性提出了质疑。
