Walters Jennifer L, Lansdell Theresa A, Lookingland Keith J, Baker Lisa E
Western Michigan University, Department of Psychology, 1903 W Michigan Ave, Kalamazoo, MI 49008-5439, United States.
Michigan State University, Department of Pharmacology and Toxicology, 1355 Bogue Street, East Lansing, MI 48824, United States.
Toxicol Appl Pharmacol. 2015 Dec 1;289(2):185-92. doi: 10.1016/j.taap.2015.09.026. Epub 2015 Oct 9.
This study sought to investigate the effects of environmentally relevant gestational followed by continued chronic exposure to the herbicide, atrazine, on motor function, cognition, and neurochemical indices of nigrostriatal dopamine (DA) activity in male rats. Dams were treated with 100 μg/kg atrazine, 10mg/kg atrazine, or vehicle on gestational day 1 through postnatal day 21. Upon weaning, male offspring continued daily vehicle or atrazine gavage treatments for an additional six months. Subjects were tested in a series of behavioral assays, and 24h after the last treatment, tissue samples from the striatum were analyzed for DA and 3,4-dihydroxyphenylacetic acid (DOPAC). At 10mg/kg, this herbicide was found to produce modest disruptions in motor functioning, and at both dose levels it significantly lowered striatal DA and DOPAC concentrations. These results suggest that exposures to atrazine have the potential to disrupt nigrostriatal DA neurons and behaviors associated with motor functioning.
本研究旨在调查孕期接触环境相关剂量、随后持续长期接触除草剂阿特拉津对雄性大鼠运动功能、认知以及黑质纹状体多巴胺（DA）活性的神经化学指标的影响。在妊娠第1天至出生后第21天，给母鼠分别施用100μg/kg阿特拉津、10mg/kg阿特拉津或赋形剂。断奶后，雄性后代继续每日接受赋形剂或阿特拉津灌胃治疗，持续6个月。对实验对象进行一系列行为测定，并在最后一次治疗24小时后，分析纹状体组织样本中的DA和3,4-二羟基苯乙酸（DOPAC）。发现10mg/kg剂量的这种除草剂会对运动功能产生适度干扰，且在两个剂量水平下，它均显著降低了纹状体DA和DOPAC浓度。这些结果表明，接触阿特拉津有可能破坏黑质纹状体DA神经元以及与运动功能相关的行为。
