Lipinska B, Rao A S, Bolten B M, Balakrishnan R, Goldberg E B
Department of Molecular Biology and Microbiology, Tufts University Medical School, Boston, Massachusetts 02111.
J Bacteriol. 1989 Jan;171(1):488-97. doi: 10.1128/jb.171.1.488-497.1989.
We sequenced bacteriophage T4 genes 2 and 3 and the putative C-terminal portion of gene 50. They were found to have appropriate open reading frames directed counterclockwise on the T4 map. Mutations in genes 2 and 64 were shown to be in the same open reading frame, which we now call gene 2. This gene codes for a protein of 27,068 daltons. The open reading frame corresponding to gene 3 codes for a protein of 20,634 daltons. Appropriate bands on polyacrylamide gels were identified at 30 and 20 kilodaltons, respectively. We found that the product of the cloned gene 2 can protect T4 DNA double-stranded ends from exonuclease V action.
我们对噬菌体T4的基因2和基因3以及推定的基因50的C末端部分进行了测序。发现它们具有在T4图谱上逆时针方向的合适开放阅读框。基因2和基因64中的突变显示在同一个开放阅读框中，我们现在将其称为基因2。该基因编码一种27,068道尔顿的蛋白质。与基因3对应的开放阅读框编码一种20,634道尔顿的蛋白质。在聚丙烯酰胺凝胶上分别在30和20千道尔顿处鉴定出合适的条带。我们发现克隆的基因2的产物可以保护T4 DNA双链末端免受核酸外切酶V的作用。
