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RNY3 调节 T 淋巴细胞模型中的细胞增殖和 IL13 mRNA 水平：IL-13 调节的一种新的可能表观遗传机制。

RNY3 modulates cell proliferation and IL13 mRNA levels in a T lymphocyte model: a possible new epigenetic mechanism of IL-13 regulation.

机构信息

Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain.

Red Cooperativa de Investigación en Salud-RETICS ARADyAL, ISCIII, Madrid, Spain.

出版信息

J Physiol Biochem. 2023 Feb;79(1):59-69. doi: 10.1007/s13105-022-00920-6. Epub 2022 Sep 12.

DOI:10.1007/s13105-022-00920-6
PMID:36089628
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9905197/
Abstract

Allergic asthma is the most common type of asthma. It is characterized by T2 cell-driven inflammation in which interleukin-13 (IL-13) plays a pivotal role. Cytoplasmic RNAs (Y-RNAs), a variety of non-coding RNAs that are dysregulated in many cancer types, are also differentially expressed in patients with allergic asthma. Their function in the development of the disease is still unknown. We investigated the potential role of RNY3 RNA (hY3) in the T2 cell inflammatory response using the Jurkat cell line as a model. hY3 expression levels were modulated to mimic the upregulation effect in allergic disease. We evaluated the effect of hY3 over cell stimulation and the expression of the T2 cytokine IL13. Total RNA was isolated and retrotranscribed, and RNA levels were assessed by qPCR. In Jurkat cells, hY3 levels increased upon stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. When transfecting with high levels of hY3 mimic molecules, cell proliferation rate decreased while IL13 mRNA levels increased upon stimulation compared to stimulated control cells. Our results show the effect of increased hY3 levels on cell proliferation and the levels of IL13 mRNA in Jurkat cells. Also, we showed that hY3 could act over other cells via exosomes. This study opens up new ways to study the potential regulatory function of hY3 over IL-13 production and its implications for asthma development.

摘要

过敏性哮喘是最常见的哮喘类型。它的特征是 T2 细胞驱动的炎症，其中白细胞介素-13（IL-13）起着关键作用。细胞质 RNA（Y-RNAs）是许多癌症类型中失调的多种非编码 RNA，在过敏性哮喘患者中也有差异表达。它们在疾病发展中的功能尚不清楚。我们使用 Jurkat 细胞系作为模型，研究了 RNY3 RNA（hY3）在 T2 细胞炎症反应中的潜在作用。通过模拟过敏疾病中的上调作用来调节 hY3 的表达水平。我们评估了 hY3 对细胞刺激和 T2 细胞因子 IL13 表达的影响。分离和逆转录总 RNA，并通过 qPCR 评估 RNA 水平。在 Jurkat 细胞中，用佛波醇 12-肉豆蔻酸 13-乙酸酯（PMA）和离子霉素刺激时，hY3 水平增加。当用高水平的 hY3 模拟分子转染时，与刺激对照细胞相比，细胞增殖率降低，而刺激时 IL13 mRNA 水平增加。我们的结果表明 hY3 水平升高对 Jurkat 细胞增殖和 IL13 mRNA 水平的影响。此外，我们还表明 hY3 可以通过外泌体作用于其他细胞。这项研究为研究 hY3 对 IL-13 产生的潜在调节功能及其对哮喘发展的影响开辟了新的途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/0052aa8b0570/13105_2022_920_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/704876fa77f3/13105_2022_920_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/38b96c0c5e28/13105_2022_920_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/69788a651d98/13105_2022_920_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/f2f75249bc6f/13105_2022_920_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/1e355c948cd1/13105_2022_920_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/0052aa8b0570/13105_2022_920_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/704876fa77f3/13105_2022_920_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/38b96c0c5e28/13105_2022_920_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/69788a651d98/13105_2022_920_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/f2f75249bc6f/13105_2022_920_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/1e355c948cd1/13105_2022_920_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b0/9905197/0052aa8b0570/13105_2022_920_Fig6_HTML.jpg

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