使用松柏醛和亚甲二氧基肉桂酸提高亚麻毛状根培养物中的木脂素含量。
Lignan enhancement in hairy root cultures of Linum album using coniferaldehyde and methylenedioxycinnamic acid.
作者信息
Ahmadian Chashmi Najmeh, Sharifi Mohsen, Behmanesh Mehrdad
机构信息
a Department of Plant Biology, Faculty of Biological Sciences , Tarbiat Modares University , Tehran , Iran.
b Department of Genetics, Faculty of Biological Sciences , Tarbiat Modares University , Tehran , Iran.
出版信息
Prep Biochem Biotechnol. 2016 Jul 3;46(5):454-60. doi: 10.1080/10826068.2015.1068802.
Feeding experiments with hairy root cultures of Linum album have established that the extracellular coniferaldehyde is a good precursor for production of two lignans: lariciresinol (LARI) and pinoresinol (PINO). The accumulation of the LARI, PINO, and podophyllotoxin (PTOX) in hairy roots were enhanced about 14.8-, 8.7-, and 1.5-fold (107.61, 8.7 and 6.42 µg g(-1) Fresh Wight), respectively, by the addition of coniferaldehyde (2 mM) to the culture media (after 24 hr). This result was correlated with an increase pinoresinol/lariciresinol reductase (PLR) expression gene and cinnamyl alcohol dehydrogenase (CAD) activity in the fed hairy roots. Adding 3,4-(methylendioxy)cinnamic acid (MDCA) precursor did not influence on the lignans accumulation, but the lignin content of the hairy roots was increased. Moreover, the expression genes of phenylalanine ammonialyase (PAL), CAD, and cinnamoyl-CoA reductase (CCR) were influenced after feeding hairy roots with MDCA.
对白亚麻毛状根培养物进行的饲喂实验表明，细胞外松柏醛是两种木脂素（落叶松脂醇（LARI）和松脂醇（PINO））合成的良好前体。在培养基中添加松柏醛（2 mM，24小时后），毛状根中LARI、PINO和鬼臼毒素（PTOX）的积累分别提高了约14.8倍、8.7倍和1.5倍（分别为107.61、8.7和6.42 µg g(-1)鲜重）。这一结果与饲喂毛状根中松脂醇/落叶松脂醇还原酶（PLR）表达基因的增加以及肉桂醇脱氢酶（CAD）活性的提高相关。添加3,4-（亚甲二氧基）肉桂酸（MDCA）前体对木脂素的积累没有影响，但毛状根的木质素含量增加。此外，用MDCA饲喂毛状根后，苯丙氨酸解氨酶（PAL）、CAD和肉桂酰辅酶A还原酶（CCR）的表达基因受到影响。
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