Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, PO Box 14115-154, Tehran, Iran.
Biotechnol Lett. 2010 Nov;32(11):1739-43. doi: 10.1007/s10529-010-0343-4. Epub 2010 Jul 7.
Treatment of Linum album cell cultures with 10 μM salicylic acid (SA) for 3 days improved podophyllotoxin (PTOX) production up to 333 μg/g dry weight (DW): over three times that of the control cultures. qPCR analyses showed that in SA-treated cells, the expression of the genes coding for phenylalanine ammonia-lyase (PAL), cinnamoyl-CoA reductase (CCR) and cinnamyl-alcohol dehydrogenase (CAD), all involved in the first steps of PTOX biosynthesis, also increased reaching a peak 8-12 h after the treatment. Expression of the pinoresinol-lariciresinol reductase gene (PLR), which is involved in one of the last biosynthetic steps, was not affected by SA. The selective action of SA on these genes can be applied to control the biotechnological production of this anticancer agent.
用 10 μM 水杨酸（SA）处理亚麻细胞培养物 3 天，可将鬼臼毒素（PTOX）的产量提高至 333μg/g 干重（DW）：是对照培养物的三倍以上。qPCR 分析表明，在经 SA 处理的细胞中，编码苯丙氨酸解氨酶（PAL）、肉桂酰辅酶 A 还原酶（CCR）和肉桂醇脱氢酶（CAD）的基因表达也增加，这些基因都参与 PTOX 生物合成的第一步，在处理后 8-12 小时达到峰值。参与最后一个生物合成步骤之一的 pinoresinol-lariciresinol 还原酶基因（PLR）不受 SA 影响。SA 对这些基因的选择性作用可应用于控制这种抗癌剂的生物技术生产。
