Chun Yong Yao, Wang Jun Kit, Tan Nguan Soon, Chan Peggy Puk Yik, Tan Timothy Thatt Yang, Choong Cleo
School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore.
Residues and Resource Reclamation Centre (R3C), Nanyang Environment and Water Research Institute (NEWRI), Nanyang Technological University, 1 Cleantech Loop, Singapore, 637141, Singapore.
Macromol Biosci. 2016 Feb;16(2):276-87. doi: 10.1002/mabi.201500258. Epub 2015 Oct 7.
A 3D injectable hydrogel-bioceramic composite consisting of gelatin-3-(4-hydroxyphenyl) propionic acid (Gtn-HPA) and carboxymethyl cellulose-tyramine (CMC-Tyr), incorporated with fish scale-derived calcium phosphate (CaP), is developed for bone applications. The hydrogel-bioceramic composite has significantly improved the elastic modulus compared to the non-filled hydrogel, of which the addition of 10 w/v% CaP showed zero order fluorescein isothiocyanate (FITC)-dextran release profile and a significantly higher proliferation rate of encapsulated cells. All the samples promote the nucleation and growth of CaP minerals when exposed to 1× SBF. Overall, the hydrogel-bioceramic composite with 10 w/v% CaP can potentially be used as a periosteum-mimicking membrane to facilitate bone regeneration.
一种由明胶 - 3 -（4 - 羟苯基）丙酸（Gtn - HPA）和羧甲基纤维素 - 酪胺（CMC - Tyr）组成，并掺入鱼鳞衍生磷酸钙（CaP）的3D可注射水凝胶 - 生物陶瓷复合材料被开发用于骨应用。与未填充的水凝胶相比，水凝胶 - 生物陶瓷复合材料的弹性模量有显著提高，其中添加10 w/v% CaP显示出零级异硫氰酸荧光素（FITC） - 葡聚糖释放曲线以及封装细胞的增殖率显著更高。当暴露于1×模拟体液（SBF）时，所有样品都能促进CaP矿物质的成核和生长。总体而言，含有10 w/v% CaP的水凝胶 - 生物陶瓷复合材料有可能用作模拟骨膜的膜来促进骨再生。
