Institute of Bioanalytical Chemistry, Saarland University, Campus B2.2, Saarbrücken, 66123, Germany.
Mass Spectrom Rev. 2017 Jan;36(1):4-15. doi: 10.1002/mas.21482. Epub 2015 Oct 7.
Tandem mass spectrometry (MS/MS) provides detailed information for structural characterization of biomolecules. The combination of electron capture dissociation (ECD) techniques with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) often provides unique ion-electron reactions and fragmentation channels in MS/MS. ECD is often a complimentary, sometimes even a superior tool to conventional MS/MS techniques. This article is aimed at providing a short overview of ECD-based fragmentation techniques (ExD) and optimization of ECD experiments for FTICR mass analyzers. Most importantly, it is meant to pique the interest of potential users for this exciting research field. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:4-15, 2017.
串联质谱（MS/MS）为生物分子的结构特征提供了详细信息。电子俘获解离（ECD）技术与傅里叶变换离子回旋共振质谱（FTICR-MS）的结合，在 MS/MS 中通常提供独特的离子-电子反应和碎裂通道。ECD 通常是一种补充手段，有时甚至是优于传统 MS/MS 技术的手段。本文旨在提供基于 ECD 的碎裂技术（ExD）的简要概述，并对 FTICR 质量分析仪的 ECD 实验进行优化。最重要的是，它旨在激发潜在用户对这一令人兴奋的研究领域的兴趣。©2015 年 Wiley 期刊，Inc. 质谱评论 36:4-15, 2017.
