Qi Yulin, Volmer Dietrich A
Institute of Bioanalytical Chemistry, Saarland University, 66123 Saarbrücken, Germany.
Analyst. 2016 Feb 7;141(3):794-806. doi: 10.1039/c5an02171e. Epub 2016 Jan 4.
Electron capture dissociation (ECD) is a tandem mass spectrometry (MS/MS) method that utilizes the interaction of ions and electrons. Its unique ability to preserve labile bonds distinguishes it from conventional threshold-based MS/MS methods, the most important of which is collision-induced dissociation (CID). During the last decade, ECD has opened up several new venues in protein analyses, for example top-down sequencing, identification of post-translational modifications, and characterization of protein-protein interactions. In recent years, a number of related dissociation techniques, so-called ExD techniques, particularly electron transfer dissociation (ETD), electron detachment dissociation (EDD), electron induced dissociation (EID), and negative electron transfer dissociation (NETD), have emerged and have extended the application range of ion-electron dissociations further. Importantly, ExD techniques have been applied beyond protein analyses, which is the focus of the current paper. This short introduction describes the application of ExD to small and medium-sized molecules and reviews important applications to natural products, biomedical compounds, synthetic molecules, crude oils, and environmental toxins.
电子捕获解离（ECD）是一种利用离子与电子相互作用的串联质谱（MS/MS）方法。它独特的保留不稳定化学键的能力使其有别于传统的基于阈值的MS/MS方法，其中最重要的是碰撞诱导解离（CID）。在过去十年中，ECD在蛋白质分析领域开辟了几个新方向，例如自上而下测序、翻译后修饰的鉴定以及蛋白质-蛋白质相互作用的表征。近年来，一些相关的解离技术，即所谓的ExD技术，特别是电子转移解离（ETD）、电子脱附解离（EDD）、电子诱导解离（EID）和负电子转移解离（NETD）相继出现，进一步扩展了离子-电子解离的应用范围。重要的是，ExD技术的应用已超越蛋白质分析领域，而这正是本文的重点。本简短介绍描述了ExD技术在中小分子中的应用，并综述了其在天然产物、生物医学化合物、合成分子、原油和环境毒素等方面的重要应用。
