miR-126在神经干细胞存活和增殖中的诱导作用。
Induction Function of miR-126 in Survival and Proliferation in Neural Stem Cells.
作者信息
Zhang Qijun, Zeng Sheng, Quan Chengyuan, Lin Xiaopo
机构信息
Department of Emergency, Pingyang People's Hospital, Wenzhou, Zhejiang, China (mainland).
出版信息
Med Sci Monit. 2015 Oct 7;21:3023-7. doi: 10.12659/MSM.894672.
BACKGROUND
The aim of this study was to investigate the potential function of miR-126 in neural stem cells (NSCs).
MATERIAL AND METHODS
Expression level of miR-126 was detected by quantitative real-time PCR (qRT-PCR). MiR-126 overexpression was established by transfecting miR-126 mimics into human NSC lines (HB1.F3 and HB1.A4 cells). Its effects on cell proliferation were studied using cell-counting kit-8 (CCK8) assay, colony formation assays. Flow cytometry was performed to evaluate the effect of miR-126 on cell survival.
RESULTS
CCK8 assay and colony formation assay showed that overexpression of miR-126 promoted cell proliferation and increased colony numbers in HB1.F3 and HB1.A4 cells. The flow cytometry confirmed the results that miR-126 inhibited cell apoptosis.
CONCLUSIONS
MiR-126 promoted the proliferation and survival of NSCs.
背景
本研究旨在探讨miR-126在神经干细胞（NSCs）中的潜在功能。
材料与方法
采用定量实时聚合酶链反应（qRT-PCR）检测miR-126的表达水平。通过将miR-126模拟物转染到人神经干细胞系（HB1.F3和HB1.A4细胞）中来实现miR-126的过表达。使用细胞计数试剂盒-8（CCK8）检测法、集落形成试验研究其对细胞增殖的影响。进行流式细胞术以评估miR-126对细胞存活的影响。
结果
CCK8检测法和集落形成试验表明，miR-126的过表达促进了HB1.F3和HB1.A4细胞的增殖并增加了集落数量。流式细胞术证实了miR-126抑制细胞凋亡的结果。
结论
miR-126促进了神经干细胞的增殖和存活。
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