通过自激活和自抑制对大肠杆菌中MarRAB操纵子的调控
Control of MarRAB Operon in Escherichia coli via Autoactivation and Autorepression.
作者信息
Prajapat Mahendra Kumar, Jain Kirti, Saini Supreet
机构信息
Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, Maharashtra, India.
Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, Maharashtra, India.
出版信息
Biophys J. 2015 Oct 6;109(7):1497-508. doi: 10.1016/j.bpj.2015.08.017.
Abstract
Choice of network topology for gene regulation has been a question of interest for a long time. How do simple and more complex topologies arise? In this work, we analyze the topology of the marRAB operon in Escherichia coli, which is associated with control of expression of genes associated with conferring resistance to low-level antibiotics to the bacterium. Among the 2102 promoters in E. coli, the marRAB promoter is the only one that encodes for an autoactivator and an autorepressor. What advantages does this topology confer to the bacterium? In this work, we demonstrate that, compared to control by a single regulator, the marRAB regulatory arrangement has the least control cost associated with modulating gene expression in response to environmental stimuli. In addition, the presence of dual regulators allows the regulon to exhibit a diverse range of dynamics, a feature that is not observed in genes controlled by a single regulator.
摘要
基因调控网络拓扑结构的选择长期以来一直是一个备受关注的问题。简单和更复杂的拓扑结构是如何产生的？在这项工作中，我们分析了大肠杆菌中marRAB操纵子的拓扑结构，它与赋予细菌对低水平抗生素抗性相关基因的表达控制有关。在大肠杆菌的2102个启动子中，marRAB启动子是唯一一个编码自激活因子和自抑制因子的启动子。这种拓扑结构赋予细菌哪些优势？在这项工作中，我们证明，与单一调节因子的控制相比，marRAB调节安排在响应环境刺激调节基因表达时具有最低的控制成本。此外，双调节因子的存在使调控子能够展现出多样的动力学特性，这是单一调节因子控制的基因所没有的特征。
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