Molina-Sánchez Pedro, Andrés Vicente
Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Vascular Biology Program, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/Melchor Fernández Almagro 3, 28029, Madrid, Spain.
Methods Mol Biol. 2015;1339:111-7. doi: 10.1007/978-1-4939-2929-0_7.
Endothelial cells (ECs) are key blood-vessel-wall components that play critical roles in the regulation of many physiological processes, including angiogenesis, coagulation, and vascular tone control, and in pathological events such as vessel inflammation and leukocyte infiltration. EC dysfunction is one of the first events associated with the development of atherosclerosis and is sustained throughout progression of the disease. The study of ECs in vitro has become an invaluable tool for investigating these vascular processes at the molecular level, and is widely used in the search for therapeutic targets and strategies. This chapter describes a protocol for the isolation and culture of primary mouse aortic ECs based on antibody-mediated EC selection.
内皮细胞（ECs）是血管壁的关键组成部分，在许多生理过程的调节中发挥着关键作用，包括血管生成、凝血和血管张力控制，以及在诸如血管炎症和白细胞浸润等病理事件中。内皮细胞功能障碍是与动脉粥样硬化发展相关的最早事件之一，并在疾病进展过程中持续存在。体外研究内皮细胞已成为在分子水平上研究这些血管过程的宝贵工具，并广泛用于寻找治疗靶点和策略。本章介绍了一种基于抗体介导的内皮细胞选择来分离和培养原代小鼠主动脉内皮细胞的方法。
