动脉粥样硬化小鼠主动脉中巨噬细胞、内皮细胞和平滑肌细胞的免疫染色
Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta.
作者信息
Menon Prashanthi, Fisher Edward A
机构信息
Marc and Ruti Bell Vascular Biology Program, Division of Cardiology, Department of Medicine, New York University School of Medicine, New York, NY, 10016, USA.
The Center for the Prevention of Cardiovascular Disease, NYU School of Medicine, Smilow 7, 522 First Avenue, New York, NY, 10016, USA.
出版信息
Methods Mol Biol. 2015;1339:131-48. doi: 10.1007/978-1-4939-2929-0_9.
Abstract
The atherosclerotic mouse aorta consists of a heterogeneous population of cells, including macrophages, endothelial cells, and smooth muscle cells that play critical roles in cardiovascular disease. Identification of these vascular cells in the vessel wall is important to understanding their function in pathological conditions. Immunohistochemistry is an invaluable technique used to detect the presence of cells in different tissues. Here, we describe immunohistochemical techniques commonly used for the detection of the vascular cells in the atherosclerotic mouse aorta using cell type-specific markers.
摘要
动脉粥样硬化小鼠主动脉由多种细胞组成，包括巨噬细胞、内皮细胞和平滑肌细胞，这些细胞在心血管疾病中起关键作用。识别血管壁中的这些血管细胞对于理解它们在病理状态下的功能很重要。免疫组织化学是一种用于检测不同组织中细胞存在的重要技术。在这里，我们描述了使用细胞类型特异性标志物检测动脉粥样硬化小鼠主动脉中血管细胞的常用免疫组织化学技术。
相似文献
1
Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta.动脉粥样硬化小鼠主动脉中巨噬细胞、内皮细胞和平滑肌细胞的免疫染色
Methods Mol Biol. 2015;1339:131-48. doi: 10.1007/978-1-4939-2929-0_9.
2
Immunostaining of Lymphocytes in Mouse Atherosclerotic Plaque.小鼠动脉粥样硬化斑块中淋巴细胞的免疫染色
Methods Mol Biol. 2015;1339:149-59. doi: 10.1007/978-1-4939-2929-0_10.
3
Deficiency of Endothelial CD40 Induces a Stable Plaque Phenotype and Limits Inflammatory Cell Recruitment to Atherosclerotic Lesions in Mice.内皮细胞 CD40 缺乏可诱导动脉粥样硬化斑块稳定表型形成并限制炎症细胞向斑块募集。
Thromb Haemost. 2021 Nov;121(11):1530-1540. doi: 10.1055/a-1397-1858. Epub 2021 May 13.
4
Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies.通过特异性抗体筛选分离小鼠原代主动脉内皮细胞。
Methods Mol Biol. 2015;1339:111-7. doi: 10.1007/978-1-4939-2929-0_7.
5
Differential Roles of Endothelial Cell-Derived and Smooth Muscle Cell-Derived Fibronectin Containing Extra Domain A in Early and Late Atherosclerosis.富含细胞外结构域 A 的内皮细胞衍生和血管平滑肌细胞衍生纤维连接蛋白在早期和晚期动脉粥样硬化中的差异作用。
Arterioscler Thromb Vasc Biol. 2020 Jul;40(7):1738-1747. doi: 10.1161/ATVBAHA.120.314459. Epub 2020 May 21.
6
CD8 T Cells Drive Plaque Smooth Muscle Cell Dedifferentiation in Experimental Atherosclerosis.CD8 T 细胞驱动实验性动脉粥样硬化斑块平滑肌细胞去分化。
Arterioscler Thromb Vasc Biol. 2024 Aug;44(8):1852-1872. doi: 10.1161/ATVBAHA.123.320084. Epub 2024 Jun 13.
7
Profiling of Primary and Mature miRNA Expression in Atherosclerosis-Associated Cell Types.动脉粥样硬化相关细胞类型中初级和成熟 miRNA 表达谱分析。
Arterioscler Thromb Vasc Biol. 2021 Jul;41(7):2149-2167. doi: 10.1161/ATVBAHA.121.315579. Epub 2021 May 13.
8
Inflammation-related induction of absent in melanoma 2 (AIM2) in vascular cells and atherosclerotic lesions suggests a role in vascular pathogenesis.血管细胞和动脉粥样硬化病变中与炎症相关的黑色素瘤缺失因子 2（AIM2）的诱导表明其在血管发病机制中的作用。
J Vasc Surg. 2014 Mar;59(3):794-803. doi: 10.1016/j.jvs.2013.03.048. Epub 2013 Jun 21.
9
NG2 Proteoglycan Ablation Reduces Foam Cell Formation and Atherogenesis via Decreased Low-Density Lipoprotein Retention by Synthetic Smooth Muscle Cells.NG2蛋白聚糖缺失通过减少合成型平滑肌细胞对低密度脂蛋白的潴留来降低泡沫细胞形成和动脉粥样硬化发生。
Arterioscler Thromb Vasc Biol. 2016 Jan;36(1):49-59. doi: 10.1161/ATVBAHA.115.306074. Epub 2015 Nov 5.
10
Differential sensitivity to apoptosis among the cells that contribute to the atherosclerotic disease.构成动脉粥样硬化疾病的细胞对细胞凋亡的敏感性差异。
Biochem Biophys Res Commun. 2007 Nov 16;363(2):444-50. doi: 10.1016/j.bbrc.2007.09.004. Epub 2007 Sep 11.
引用本文的文献
1
Sterol carrier protein 2: A promising target in the pathogenesis of atherosclerosis.固醇载体蛋白2：动脉粥样硬化发病机制中的一个有前景的靶点。
Genes Dis. 2022 Jan 10;10(2):457-467. doi: 10.1016/j.gendis.2021.12.007. eCollection 2023 Mar.
2
β3 adrenergic agonism: A novel pathway which improves right ventricular-pulmonary arterial hemodynamics in pulmonary arterial hypertension.β3 肾上腺素能激动剂：改善肺动脉高压患者右心室-肺动脉血液动力学的新途径。
Physiol Rep. 2023 Jan;11(1):e15549. doi: 10.14814/phy2.15549.
3
Interleukin-22: a potential therapeutic target in atherosclerosis.白细胞介素-22：动脉粥样硬化的潜在治疗靶点。
Mol Med. 2021 Aug 13;27(1):88. doi: 10.1186/s10020-021-00353-9.
4
Endothelial Rap1 (Ras-Association Proximate 1) Restricts Inflammatory Signaling to Protect From the Progression of Atherosclerosis.内皮细胞 Rap1（Ras 相关蛋白临近 1）限制炎症信号传递以防止动脉粥样硬化进展。
Arterioscler Thromb Vasc Biol. 2021 Feb;41(2):638-650. doi: 10.1161/ATVBAHA.120.315401. Epub 2020 Dec 3.
5
Tripartite factors leading to molecular divergence between human and murine smooth muscle.导致人类和鼠类平滑肌分子分化的三方因素。
PLoS One. 2020 Jan 16;15(1):e0227672. doi: 10.1371/journal.pone.0227672. eCollection 2020.
6
Selenomethionine supplementation reduces lesion burden, improves vessel function and modulates the inflammatory response within the setting of atherosclerosis.硒蛋氨酸补充可减少动脉粥样硬化病变负担，改善血管功能，并调节炎症反应。
Redox Biol. 2020 Jan;29:101409. doi: 10.1016/j.redox.2019.101409. Epub 2019 Dec 16.
7
Identification of a FGF18-expressing alveolar myofibroblast that is developmentally cleared during alveologenesis.鉴定出一种表达 FGF18 的肺泡肌成纤维细胞，其在肺泡发生过程中被清除。
Development. 2020 Jan 17;147(2):dev181032. doi: 10.1242/dev.181032.
8
Single-Cell RNA Sequencing of Visceral Adipose Tissue Leukocytes Reveals that Caloric Restriction Following Obesity Promotes the Accumulation of a Distinct Macrophage Population with Features of Phagocytic Cells.内脏脂肪组织白细胞的单细胞RNA测序表明，肥胖后进行热量限制会促进具有吞噬细胞特征的独特巨噬细胞群体的积累。
Immunometabolism. 2019;1. doi: 10.20900/immunometab20190008. Epub 2019 Jul 19.
9
Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution.研究单核细胞和巨噬细胞在动脉粥样硬化进展与消退过程中迁移的方法。
Methods Mol Biol. 2019;1951:153-165. doi: 10.1007/978-1-4939-9130-3_12.
本文引用的文献
1
Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages.小鼠组织巨噬细胞的特征和多样性所依赖的基因表达谱和转录调控途径。
Nat Immunol. 2012 Nov;13(11):1118-28. doi: 10.1038/ni.2419. Epub 2012 Sep 30.
2
Whole animal perfusion fixation for rodents.啮齿动物的全动物灌注固定
J Vis Exp. 2012 Jul 30(65):3564. doi: 10.3791/3564.
3
HDL promotes rapid atherosclerosis regression in mice and alters inflammatory properties of plaque monocyte-derived cells.高密度脂蛋白可促进小鼠动脉粥样硬化快速消退，并改变斑块单核细胞来源细胞的炎症特性。
Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7166-71. doi: 10.1073/pnas.1016086108. Epub 2011 Apr 11.
4
Principles and methods of immunohistochemistry.免疫组织化学的原理与方法。
Methods Mol Biol. 2011;691:83-96. doi: 10.1007/978-1-60761-849-2_5.
5
Molecular determinants of vascular smooth muscle cell diversity.血管平滑肌细胞多样性的分子决定因素。
Circ Res. 2005 Feb 18;96(3):280-91. doi: 10.1161/01.RES.0000155951.62152.2e.
6
Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro.内皮标志物PECAM-1、血管性血友病因子（vWf）和CD34在体内和体外的表达。
Exp Mol Pathol. 2002 Jun;72(3):221-9. doi: 10.1006/exmp.2002.2424.
7
Laser capture microdissection analysis of gene expression in macrophages from atherosclerotic lesions of apolipoprotein E-deficient mice.载脂蛋白E缺陷小鼠动脉粥样硬化病变巨噬细胞基因表达的激光捕获显微切割分析
Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2234-9. doi: 10.1073/pnas.042683999. Epub 2002 Feb 12.
8
Elevating high-density lipoprotein cholesterol in apolipoprotein E-deficient mice remodels advanced atherosclerotic lesions by decreasing macrophage and increasing smooth muscle cell content.提高载脂蛋白E缺乏小鼠的高密度脂蛋白胆固醇水平，可通过减少巨噬细胞并增加平滑肌细胞含量来重塑晚期动脉粥样硬化病变。
Circulation. 2001 Nov 13;104(20):2447-52. doi: 10.1161/hc4501.098952.
9
Mouse models of atherosclerosis.动脉粥样硬化的小鼠模型。
Science. 1996 May 3;272(5262):685-8. doi: 10.1126/science.272.5262.685.
10
Atherosclerosis in mice lacking apo E. Evaluation of lesional development and progression.缺乏载脂蛋白E的小鼠的动脉粥样硬化。病变发展和进展的评估。
Arterioscler Thromb. 1994 Jan;14(1):141-7. doi: 10.1161/01.atv.14.1.141.
Zotero 插件