小鼠动脉粥样硬化斑块中淋巴细胞的免疫染色

Immunostaining of Lymphocytes in Mouse Atherosclerotic Plaque.

作者信息

Gisterå Anton, Ketelhuth Daniel F J

机构信息

Center for Molecular Medicine, Department of Medicine, Karolinska University Hospital, Karolinska Institute, SE-17176, Stockholm, Sweden.

出版信息

Methods Mol Biol. 2015;1339:149-59. doi: 10.1007/978-1-4939-2929-0_10.

DOI:10.1007/978-1-4939-2929-0_10

PMID:26445787

Abstract

Immunostaining can be defined as a method for identification of constituents in situ, e.g., proteins, using specific antigen-antibody interactions, visualized by color or fluorescence. In experimental research of atherosclerosis, immunostaining is a key method used for the characterization of the cellular composition in the atherosclerotic plaques. This includes the quantification of macrophages, smooth muscle cells, and lymphocytes-especially T lymphocytes. In this chapter we focus on basic protocols for characterization of lymphocytes and/or local T lymphocyte-related antigens in mouse atherosclerotic lesions.

摘要

免疫染色可定义为一种利用特异性抗原 - 抗体相互作用原位鉴定成分（如蛋白质）的方法，通过颜色或荧光进行可视化。在动脉粥样硬化的实验研究中，免疫染色是用于表征动脉粥样硬化斑块中细胞组成的关键方法。这包括对巨噬细胞、平滑肌细胞和淋巴细胞（尤其是T淋巴细胞）进行定量。在本章中，我们重点介绍在小鼠动脉粥样硬化病变中表征淋巴细胞和/或局部T淋巴细胞相关抗原的基本方案。