Arc和Mnt阻遏物的DNA结合特异性由N端残基的一个短区域决定。
DNA binding specificity of the Arc and Mnt repressors is determined by a short region of N-terminal residues.
作者信息
Knight K L, Sauer R T
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
Proc Natl Acad Sci U S A. 1989 Feb;86(3):797-801. doi: 10.1073/pnas.86.3.797.
Abstract
The Arc and Mnt repressors of phage P22 are related proteins that bind to different operator DNA sites. By creating a hybrid Arc-Mnt protein, we show that the binding specificity of Mnt can be switched to that of Arc by replacing six residues at the N terminus of Mnt with the corresponding nine residues from Arc.
摘要
噬菌体P22的Arc和Mnt阻遏物是相关蛋白，它们与不同的操纵基因DNA位点结合。通过构建一种Arc-Mnt杂合蛋白，我们发现，将Mnt N端的六个残基替换为Arc相应的九个残基后，Mnt的结合特异性可转变为Arc的结合特异性。
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