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Identification of resonances from an oncogenic activating locus of human N-RAS-encoded p21 protein using isotope-edited NMR.使用同位素编辑核磁共振技术鉴定人N-RAS编码的p21蛋白致癌激活位点的共振峰。
Proc Natl Acad Sci U S A. 1989 Feb;86(3):817-20. doi: 10.1073/pnas.86.3.817.
2
Structural and dynamic differences between normal and transforming N-ras gene products: a 31P and isotope-edited 1H NMR study.正常与转化型N-ras基因产物之间的结构和动力学差异：一项31P和同位素编辑1H NMR研究。
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NMR study of the phosphoryl binding loop in purine nucleotide proteins: evidence for strong hydrogen bonding in human N-ras p21.
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Proton NMR measurements of bacteriophage T4 lysozyme aided by 15N isotopic labeling: structural and dynamic studies of larger proteins.通过15N同位素标记辅助的噬菌体T4溶菌酶的质子核磁共振测量：较大蛋白质的结构与动力学研究
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NMR studies of the conformational change in human N-p21ras produced by replacement of bound GDP with the GTP analog GTP gamma S.用GTP类似物GTPγS取代结合的GDP所引发的人N-p21ras构象变化的核磁共振研究。
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A 1H-15N NMR study of human c-Ha-ras protein: biosynthetic incorporation of 15N-labeled amino acids.人源c-Ha-ras蛋白的1H-15N核磁共振研究：15N标记氨基酸的生物合成掺入
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Protein expression, selective isotopic labeling, and analysis of hyperfine-shifted NMR signals of Anabaena 7120 vegetative [2Fe-2S]ferredoxin.鱼腥藻7120营养型[2Fe-2S]铁氧化还原蛋白的蛋白质表达、选择性同位素标记及超精细位移核磁共振信号分析。
Arch Biochem Biophys. 1995 Jan 10;316(1):619-34. doi: 10.1006/abbi.1995.1082.

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Data requirements for reliable chemical shift assignments in deuterated proteins.
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Biochem J. 2000 Mar 1;346 Pt 2(Pt 2):501-8.
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Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8160-6. doi: 10.1073/pnas.93.16.8160.
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Sequence-specific 1H and 15N resonance assignments and secondary structure of GDP-bound human c-Ha-Ras protein in solution.溶液中GDP结合的人c-Ha-Ras蛋白的序列特异性1H和15N共振归属及二级结构
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8
A 1H-15N NMR study of human c-Ha-ras protein: biosynthetic incorporation of 15N-labeled amino acids.人源c-Ha-ras蛋白的1H-15N核磁共振研究：15N标记氨基酸的生物合成掺入
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9
Sequential assignment of the backbone nuclei (1H, 15N and 13C) of c-H-ras p21 (1-166).GDP using a novel 4D NMR strategy.使用一种新型的4D NMR策略对c-H-ras p21（1-166）.GDP的主链核（1H、15N和13C）进行顺序归属。
J Biomol NMR. 1992 Nov;2(6):639-46. doi: 10.1007/BF02192852.

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Assignment of the three methionyl carbonyl carbon resonances in Streptomyces subtilisin inhibitor by a carbon-13 and nitrogen-15 double-labeling technique. A new strategy for structural studies of proteins in solution.通过碳-13和氮-15双标记技术对枯草芽孢杆菌蛋白酶抑制剂中三个甲硫氨酰羰基碳共振峰的归属。一种用于溶液中蛋白质结构研究的新策略。
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A model for the tertiary structure of p21, the product of the ras oncogene.一种关于ras癌基因产物p21三级结构的模型。
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3
Structure of the GDP domain of EF-Tu and location of the amino acids homologous to ras oncogene proteins.EF-Tu的GDP结构域的结构以及与ras癌基因蛋白同源的氨基酸的位置。
Science. 1985 Oct 4;230(4721):32-6. doi: 10.1126/science.3898365.
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Nuclear magnetic resonance observation and dynamics of specific amide protons in T4 lysozyme.T4溶菌酶中特定酰胺质子的核磁共振观测与动力学
Biochemistry. 1985 Feb 12;24(4):817-22. doi: 10.1021/bi00325a001.
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Biochemical and biological properties of the human N-ras p21 protein.人N-ras p21蛋白的生化及生物学特性
Mol Cell Biol. 1987 Jan;7(1):541-4. doi: 10.1128/mcb.7.1.541-544.1987.
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ras genes.RAS基因
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NMR sequential assignment of Escherichia coli thioredoxin utilizing random fractional deuteriation.利用随机部分氘代对大肠杆菌硫氧还蛋白进行核磁共振序列归属
Biochemistry. 1988 Jan 12;27(1):142-50. doi: 10.1021/bi00401a022.
8
Biochemical properties of a highly purified v-rasH p21 protein overproduced in Escherichia coli and inhibition of its activities by a monoclonal antibody.在大肠杆菌中过量产生的高度纯化的v-rasH p21蛋白的生化特性及其活性被单克隆抗体抑制的情况
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9
Proton NMR measurements of bacteriophage T4 lysozyme aided by 15N isotopic labeling: structural and dynamic studies of larger proteins.通过15N同位素标记辅助的噬菌体T4溶菌酶的质子核磁共振测量：较大蛋白质的结构与动力学研究
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1244-8. doi: 10.1073/pnas.84.5.1244.
10
Ha-ras proteins exhibit GTPase activity: point mutations that activate Ha-ras gene products result in decreased GTPase activity.Ha-ras蛋白具有GTP酶活性：激活Ha-ras基因产物的点突变会导致GTP酶活性降低。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):376-80. doi: 10.1073/pnas.82.2.376.

使用同位素编辑核磁共振技术鉴定人N-RAS编码的p21蛋白致癌激活位点的共振峰。

Identification of resonances from an oncogenic activating locus of human N-RAS-encoded p21 protein using isotope-edited NMR.

作者信息

Burk S C, Papastavros M Z, McCormick F, Redfield A G

机构信息

Department of Biochemistry, Brandeis University, Waltham, MA 02254.

出版信息

Proc Natl Acad Sci U S A. 1989 Feb;86(3):817-20. doi: 10.1073/pnas.86.3.817.

DOI:10.1073/pnas.86.3.817
PMID:2644645
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC286568/
Abstract

A sample of Escherichia coli-expressed human N-RAS-encoded p21, a 21-kDa protein, was selectively labeled with 15N at each of the 14 glycine amide positions. Two-dimensional proton-observe 15N correlation spectra showed one peak for each glycine residue. Five glycine resonances were identified with residues near the nucleotide binding site and provide useful reporters of several oncogene-activating positions. Three of these resonances were assigned to residues 10, 15, and 115 from the spectrum of a sample that was also labeled with [13C]valine. These resonances showed extra splitting or broadening due to the 13C label, which could be eliminated by 13C decoupling. Two other peaks were unambiguously identified as Gly-12 and Gly-13 using a one-dimensional edited nuclear Overhauser experiment and by spectral comparison with an Asp-12 mutant. These assignments have provided several site-specific probes of critical domains in p21.

摘要

一份由大肠杆菌表达的人N-RAS编码的p21（一种21 kDa的蛋白质）样本，在14个甘氨酰胺位置上均被15N选择性标记。二维质子观测15N相关谱显示每个甘氨酸残基有一个峰。五个甘氨酸共振峰在核苷酸结合位点附近被鉴定出来，它们为几个致癌基因激活位点提供了有用的报告信号。其中三个共振峰从同样用[13C]缬氨酸标记的样本光谱中被指定为第10、15和115位残基。这些共振峰由于13C标记而显示出额外的分裂或展宽，通过13C去耦可以消除这种现象。使用一维编辑核Overhauser实验并通过与Asp-12突变体的光谱比较，另外两个峰被明确鉴定为Gly-12和Gly-13。这些归属为p21关键结构域提供了几个位点特异性探针。