Garige Mamatha, Walters Eric
Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, DC, USA.
Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, DC, USA.
Biochem Biophys Res Commun. 2015 Nov 13;467(2):275-81. doi: 10.1016/j.bbrc.2015.09.175. Epub 2015 Oct 9.
The molecular basis for nutraceutical properties of the polyphenol curcumin (Curcuma longa, Turmeric) is complex, affecting multiple factors that regulate cell signaling and homeostasis. Here, we report the effect of curcumin on cellular and developmental mechanisms in the eukaryotic model, Dictyostelium discoideum. Dictyostelium proliferation was inhibited in the presence of curcumin, which also suppressed the prestarvation marker, discoidin I, members of the yakA-mediated developmental signaling pathway, and expression of the extracellular matrix/cell adhesion proteins (DdCAD and csA). This resulted in delayed chemotaxis, adhesion, and development of the organism. In contrast to the inhibitory effects on developmental genes, curcumin induced gstA gene expression, overall GST activity, and generated production of reactive oxygen species. These studies expand our knowledge of developmental and biochemical signaling influenced by curcumin, and lends greater consideration of GST enzyme function in eukaryotic cell signaling, development, and differentiation.
多酚类姜黄素（姜黄属植物姜黄）的营养保健特性的分子基础十分复杂，它会影响调节细胞信号传导和体内平衡的多个因素。在此，我们报告了姜黄素对真核模型盘基网柄菌细胞和发育机制的影响。在姜黄素存在的情况下，盘基网柄菌的增殖受到抑制，同时姜黄素还抑制了饥饿前标志物盘状球蛋白I、yakA介导的发育信号通路成员以及细胞外基质/细胞粘附蛋白（DdCAD和csA）的表达。这导致了生物体趋化性、粘附和发育的延迟。与对发育基因的抑制作用相反，姜黄素诱导了gstA基因表达、总体谷胱甘肽S-转移酶（GST）活性，并产生活性氧。这些研究扩展了我们对受姜黄素影响的发育和生化信号传导的认识，并促使人们更多地考虑GST酶在真核细胞信号传导、发育和分化中的功能。
