大肠杆菌O160含磷酸糖基O-多糖生物合成的结构与遗传学
Structure and genetics of biosynthesis of the glycosyl phosphate-containing O-polysaccharide of Escherichia coli O160.
作者信息
Perepelov Andrei V, Guo Xi, Senchenkova Sof'ya N, Shashkov Alexander S, Knirel Yuriy A
机构信息
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China.
出版信息
Carbohydr Res. 2015 Nov 19;417:89-93. doi: 10.1016/j.carres.2015.09.007. Epub 2015 Sep 16.
On mild acid degradation of the lipopolysaccharide of Escherichia coli O160, the O-polysaccharide was cleaved by acid-labile glycosyl phosphate linkages in the main chain. The resultant oligosaccharide and the alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)H and (13)C NMR spectroscopies, and the following structure of the branched pentasaccharide repeating unit of the O-polysaccharide was established: The O-antigen gene cluster of E. coli O160 was found to be consistent with the O-polysaccharide structure established.
对大肠杆菌O160脂多糖进行温和酸降解时，O-多糖通过主链中对酸不稳定的糖基磷酸酯键断裂。通过糖分析以及¹H和¹³C NMR光谱对所得寡糖和碱处理的脂多糖进行了研究，并确定了O-多糖支链五糖重复单元的以下结构：发现大肠杆菌O160的O抗原基因簇与所确定的O-多糖结构一致。
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