大肠杆菌 O76 0 抗原的结构和基因簇。
Structure and gene cluster of the O-antigen of Escherichia coli O76.
机构信息
ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
出版信息
Carbohydr Res. 2013 Aug 9;377:14-7. doi: 10.1016/j.carres.2013.04.027. Epub 2013 Apr 30.
The O-polysaccharide (O-antigen) of Escherichia coli O76 was studied by sugar analysis along with 1D and 2D (1)H,(13)C NMR spectroscopies. The following structure of the linear tetrasaccharide repeating unit was established: →4)-β-D-GlcpA-(1→4)-β-D-GalpNAc3Ac-(1→4)-α-D-GalpNAc-(1→3)-β-D-GalpNAc-(1→. The degree of O-acetylation of 4-substituted β-GalNAc residue is ~70%. The O-antigen gene cluster of E. coli O76 was sequenced. The functions of genes in the O-antigen gene cluster were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the E. coli O76 O-antigen structure.
大肠杆菌 O76 的 O-多糖（O-抗原）通过糖分析以及 1D 和 2D（1）H、（13）C NMR 光谱进行了研究。建立了线性四糖重复单元的以下结构：→4）-β-D-GlcpA-（1→4）-β-D-GalpNAc3Ac-（1→4）-α-D-GalpNAc-（1→3）-β-D-GalpNAc-（1→。4 位取代β-GalNAc 残基的 O-乙酰化程度约为 70%。大肠杆菌 O76 的 O-抗原基因簇进行了测序。通过与现有数据库中的序列进行比较，推测 O-抗原基因簇中基因的功能，并与大肠杆菌 O76 O-抗原结构完全一致。
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