Senchenkova Sof'ya N, Guo Xi, Filatov Andrei V, Perepelov Andrei V, Liu Bin, Shashkov Alexander S, Knirel Yuriy A
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457, Tianjin, PR China.
Carbohydr Res. 2016 Sep 2;432:83-7. doi: 10.1016/j.carres.2016.07.011. Epub 2016 Jul 14.
Mild alkaline degradation of the lipopolysaccharide of Escherichia coli O80 afforded a polysaccharide, which was studied by sugar analysis, selective cleavage of glycosidic linkages, and (1)H and (13)C NMR spectroscopy. Solvolysis of the polysaccharide with CF3CO2H cleaved the linkages of α-Fuc and β-linked GlcNAc and GalNAc residues to give two disaccharides. The following structure of the hexasaccharide repeating unit of the O-polysaccharide was established: The polysaccharide repeat also contains a minor O-acetyl group but its position was not determined. The O-antigen gene cluster of E. coli O80 between the conserved galF and gnd genes was analyzed and found to be consistent with the O-polysaccharide structure established.
大肠杆菌O80脂多糖的温和碱性降解产生了一种多糖，通过糖分析、糖苷键的选择性裂解以及¹H和¹³C核磁共振光谱对其进行了研究。用CF₃CO₂H对该多糖进行溶剂解，裂解了α-岩藻糖以及β-连接的N-乙酰葡糖胺和N-乙酰半乳糖胺残基的键，生成了两种二糖。确定了O-多糖六糖重复单元的以下结构：该多糖重复单元还含有一个较小的O-乙酰基，但未确定其位置。对大肠杆菌O80位于保守的galF和gnd基因之间的O抗原基因簇进行了分析，发现与所确定的O-多糖结构一致。
