Fiedoruk-Pogrebniak Marta, Koncki Robert
University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
Talanta. 2015 Nov 1;144:184-8. doi: 10.1016/j.talanta.2015.06.001. Epub 2015 Jun 3.
A bianalyte multicommutated flow analysis (MCFA) system allowing simultaneous determination of calcium and phosphate ions is developed. The detection of ions is based on measurements of fluorescence of calcein and rhodamine B, respectively. For such measurements performed under flow analysis conditions two dedicated detectors made of three integrated light emitting diodes each and operating according to fluorometric paired emitter detector diode (FPEDD) principle, have been used. The developed 2FPEDD-MCFA system has been applied for serum analysis. The system provides low detection limits (16 µM and 3.6 µM for calcium and phosphate ions, respectively) and therefore small consumption of sample (8 µL) for analysis. The throughput of the system allows about 20 bianalyte determinations per hour. The presented system was validated using human serum samples. The analysis results show good correlation with those from the clinical laboratory obtained using reference photometric methods. The results of real samples analysis show that it is necessary to determine both calcium and phosphate ions simultaneously because their levels is serum are rather weakly correlated.
开发了一种能够同时测定钙离子和磷酸根离子的双分析物多通道流动分析（MCFA）系统。离子检测分别基于钙黄绿素和罗丹明B荧光的测量。对于在流动分析条件下进行的此类测量，使用了两个专用探测器，每个探测器由三个集成发光二极管组成，并根据荧光配对发射体探测器二极管（FPEDD）原理运行。所开发的2FPEDD-MCFA系统已应用于血清分析。该系统具有低检测限（钙离子和磷酸根离子分别为16 μM和3.6 μM），因此分析时样品消耗量小（8 μL）。系统的通量允许每小时进行约20次双分析物测定。使用人血清样品对所展示的系统进行了验证。分析结果与临床实验室使用参考光度法获得的结果具有良好的相关性。实际样品分析结果表明，有必要同时测定钙离子和磷酸根离子，因为它们在血清中的水平相关性较弱。
