University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
Talanta. 2012 May 15;93:106-10. doi: 10.1016/j.talanta.2012.01.055. Epub 2012 Feb 2.
A low-cost and compact complete fluorometric detector dedicated for measurements under conditions of flow injection analysis has been developed. This device is fabricated by integration of light emitting diodes (LEDs) applied in the double role: as the source of light inducing fluorescence and as the detector of light emitted by excited fluorescent analyte. The device is made of three LEDs only, without any additional fibers, filters and lenses. The LEDs are integrated in the form of flow cell of 0.060 mL internal volume. The developed detector has been tested in simple flow injection manifold dedicated for fluorometric determination of calcium using calcein method. The system offers sensitive and selective determination of calcium at ppm levels with relatively high flow throughput (near 60 injections per hour) and satisfactory reproducibility. The practical utility of developed detector has been confirmed by its application for analysis of mineral waters, medicines as well as physiological fluids.
已经开发出一种低成本、紧凑的全荧光检测器，专门用于流动注射分析条件下的测量。该设备通过集成发光二极管 (LED) 制成，这些 LED 具有双重作用：既是激发荧光的光源，也是被激发荧光分析物发射光的探测器。该设备仅由三个 LED 组成，没有任何额外的光纤、滤光片和透镜。这些 LED 以 0.060 毫升内部体积的流动池形式集成在一起。已经在简单的流动注射流路中对开发的检测器进行了测试，该流路专门用于使用钙黄绿素法进行荧光法测定钙。该系统提供了对钙的灵敏和选择性测定，可达到 ppm 水平，具有相对较高的流量（近每小时 60 次注射）和令人满意的重现性。通过将其应用于矿泉水、药物以及生理流体的分析，已经证实了所开发的检测器的实际用途。
